Simultaneous production of multiple antimicrobial compounds by Bacillus velezensis ML122-2 isolated from Assam tea leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]

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Date
2021
Authors
Rungsirivanich, Patthanasak
Parlindungan, Elvina
O’Connor, Paula M.
Field, Des
Mahony, Jennifer
Thongwai, Narumol
van Sinderen, Douwe
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Frontiers Media S.A.
Published Version
10.3389/fmicb.2021.789362
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Abstract
Bacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea.
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Keywords
Amylocyclicin , Bacteriocin , Gene cluster , Biocontrol , Miang , Plipastatin , RT-qPCR , Surfactin
Citation
Rungsirivanich, P., Parlindungan, E., O’Connor, P.M., Field, D., Mahony, J., Thongwai, N. and Van Sinderen, D. (2021) ‘Simultaneous production of multiple antimicrobial compounds by bacillus velezensis ml122-2 isolated from assam tea leaf [camellia sinensis var. Assamica (J. W. Mast.) kitam. ]’, Frontiers in Microbiology, 12, 789362 (14pp). doi: 10.3389/fmicb.2021.789362
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https://hdl.handle.net/10468/15251
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APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles
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©2021 Rungsirivanich, Parlindungan, O’Connor, Field, Mahony, Thongwai and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.