Simultaneous production of multiple antimicrobial compounds by Bacillus velezensis ML122-2 isolated from Assam tea leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]

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dc.contributor.authorRungsirivanich, Patthanasaken
dc.contributor.authorParlindungan, Elvinaen
dc.contributor.authorO’Connor, Paula M.en
dc.contributor.authorField, Desen
dc.contributor.authorMahony, Jenniferen
dc.contributor.authorThongwai, Narumolen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.funderNational Research Council of Thailanden
dc.contributor.funderTea Gallery Group (Thailand) Co., Ltd.en
dc.contributor.funderAmazing Tea Limited Partnershipen
dc.contributor.funderGraduate School, Chiang Mai Universityen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-11-21T14:59:57Z
dc.date.available2023-11-21T14:59:57Z
dc.date.issued2021en
dc.description.abstractBacillus velezensis ML122-2 is an antimicrobial-producing strain isolated from the leaf of Assam tea or Miang [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]. The cell-free supernatant (CFS) of strain ML122-2 exhibits a broad-spectrum antimicrobial activity against various Gram-positive and Gram-negative bacteria as well as the mold Penicillium expansum. The genome of B. velezensis ML122-2 was sequenced and in silico analysis identified three potential bacteriocin-associated gene clusters, that is, those involved in the production of mersacidin, amylocyclicin, and LCI. Furthermore, six gene clusters exhibiting homology (75–100% DNA sequence identity) to those associated with the secondary metabolites bacilysin, bacillibactin, surfactin, macrolactin H, bacillaene, and plipastatin were identified. Individual antimicrobial activities produced by B. velezensis ML122-2 were purified and characterized by Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis, revealing three antimicrobial peptides with molecular masses corresponding to surfactin, plipastatin, and amylocyclicin. Transcriptional analysis of specific genes associated with mersacidin (mrsA), amylocyclicin (acnA), plipastatin (ppsA), and surfactin (srfAA) production by B. velezensis ML122-2 showed that the first was not transcribed under the conditions tested, while the latter three were consistent with the presence of the associated peptides as determined by mass spectrometry analysis. These findings demonstrate that B. velezensis ML122-2 has the genetic capacity to produce a wide range of antimicrobial activities that may support a specific community structure and highlight the biotechnological properties of Assam tea.en
dc.description.sponsorshipNational Research Council of Thailand (Grant number PHD60I0089); Graduate School, Chiang Mai University (Scholarship for Teaching Assistant and Research Assistant (TA/RA))en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid789362en
dc.identifier.citationRungsirivanich, P., Parlindungan, E., O’Connor, P.M., Field, D., Mahony, J., Thongwai, N. and Van Sinderen, D. (2021) ‘Simultaneous production of multiple antimicrobial compounds by bacillus velezensis ml122-2 isolated from assam tea leaf [camellia sinensis var. Assamica (J. W. Mast.) kitam. ]’, Frontiers in Microbiology, 12, 789362 (14pp). doi: 10.3389/fmicb.2021.789362en
dc.identifier.doi10.3389/fmicb.2021.789362en
dc.identifier.endpage14en
dc.identifier.issn1664-302Xen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15251
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.ispartofFrontiers in Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights©2021 Rungsirivanich, Parlindungan, O’Connor, Field, Mahony, Thongwai and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAmylocyclicinen
dc.subjectBacteriocinen
dc.subjectGene clusteren
dc.subjectBiocontrolen
dc.subjectMiangen
dc.subjectPlipastatinen
dc.subjectRT-qPCRen
dc.subjectSurfactinen
dc.titleSimultaneous production of multiple antimicrobial compounds by Bacillus velezensis ML122-2 isolated from Assam tea leaf [Camellia sinensis var. assamica (J.W.Mast.) Kitam.]en
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.volume12en
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