Simulated gastrointestinal digestion of nisin and interaction between nisin and bile

Simple item page
dc.contributor.authorGough, Ronan
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorRea, Mary C.
dc.contributor.authorGómez-Sala, Beatriz
dc.contributor.authorMiao, Song
dc.contributor.authorHill, Colin
dc.contributor.authorBrodkorb, André
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderTeagascen
dc.date.accessioned2017-09-22T10:54:50Z
dc.date.available2017-09-22T10:54:50Z
dc.date.issued2017-08-14
dc.date.updated2017-09-22T10:43:06Z
dc.description.abstractNisin, an antimicrobial peptide showing activity against many Gram positive bacteria, is widely used as a food preservative. The simulated gastrointestinal digestion of nisin (variant A) was studied using the in vitro INFOGEST digestion method. Following oral, gastric and small intestinal digestion, there was no intact nisin in the system and the nisin was primarily digested by pancreatin. After digestion, six nisin fragments (1–11, 1–12, 1–20, 1–21, 1–29 and 1–32) were identified by reversed phase high performance liquid chromatography and mass spectroscopy and four of these nisin fragments (1–20, 1–21, 1–29 and 1–32) demonstrated low antibacterial activity against Lactococcus lactis HP in agar diffusion activity assays. Additionally, it was observed that bile salts form a complex with nisin. This was examined by atomic force microscopy, turbidity and dynamic light scattering, which showed that this interaction resulted in significantly larger bile salt micelles. The presence of bile salts at physiological levels significantly altered the relative amounts of the nisin fragments 1–12, 1–20 and 1–29 produced during an in vitro digestion. This study highlights the importance of including bile in simulated digestions of antimicrobial peptides in order to obtain a more accurate simulation of the in vivo digestion products and their activity.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine (FIRM Programme Grant Number 10/RD/TMFRC/701); Teagasc (Walsh Fellowship Grant Number 2012221)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGough, R., O'Connor, P. M., Rea, M. C., Gómez-Sala , B., Miao, S., Hill, C. and Brodkorb, A (2017) 'Simulated gastrointestinal digestion of nisin and interaction between nisin and bile', LWT - Food Science and Technology, 86, pp. 530-537. doi:10.1016/j.lwt.2017.08.031en
dc.identifier.doi10.1016/j.lwt.2017.08.031
dc.identifier.endpage537en
dc.identifier.issn0023-6438
dc.identifier.journaltitleLWT - Food Science and Technologyen
dc.identifier.startpage530en
dc.identifier.urihttps://hdl.handle.net/10468/4774
dc.identifier.volume86en
dc.language.isoenen
dc.publisherElsevier Ltd.en
dc.rights© 2017, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectNisinen
dc.subjectDigestionen
dc.subjectBileen
dc.subjectAntimicrobial peptideen
dc.subjectSurfactanten
dc.titleSimulated gastrointestinal digestion of nisin and interaction between nisin and bileen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
3698.pdf
Size:
1.42 MB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles