dc.contributor.author	Culligan, Eamonn P.
dc.contributor.author	Sleator, Roy D.
dc.contributor.author	Marchesi, Julian R.
dc.contributor.author	Hill, Colin
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	APC Microbiome Institute, College of Medicine and Health, University College Cork
dc.contributor.funder	Royal Society, United Kingdom
dc.contributor.funder	European Commission
dc.contributor.funder	European Society of Clinical Microbiology and Infectious Diseases, Switzerland
dc.date.accessioned	2016-02-17T11:43:39Z
dc.date.available	2016-02-17T11:43:39Z
dc.date.issued	2014
dc.description.abstract	The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.	en
dc.description.sponsorship	Science Foundation Ireland (SFI Grant 07/CE/B1368); European Society of Clinical Microbiology and Infectious Diseases, Switzerland (Research Fellowship); European Commission (EU FP7 IAPP project ClouDx-i)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e103318
dc.identifier.citation	Culligan EP, Sleator RD, Marchesi JR, Hill C (2014) Metagenomic Identification of a Novel Salt Tolerance Gene from the Human Gut Microbiome Which Encodes a Membrane Protein with Homology to a brp/blh-Family β-Carotene 15,15′-Monooxygenase. PLoS ONE 9(7): e103318. doi:10.1371/journal.pone.0103318
dc.identifier.doi	10.1371/journal.pone.0103318
dc.identifier.issn	1932-6203
dc.identifier.issued	7	en
dc.identifier.journaltitle	PLOS ONE	en
dc.identifier.uri	https://hdl.handle.net/10468/2331
dc.identifier.volume	9	en
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2015 Culligan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	en
dc.subject	Escherichia coli	en
dc.subject	Listeria monocytogenes	en
dc.subject	Stress response	en
dc.subject	Gastrointestinal tract	en
dc.subject	Staphylococcus aureus	en
dc.subject	Sequence alignments	en
dc.subject	Bacterial stress	en
dc.subject	Oxidative stress	en
dc.subject	Binding protein	en
dc.subject	Sigma-Factor	en
dc.title	Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-carotene 15,15'-monooxygenase	en
dc.type	Article (peer-reviewed)	en

Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-carotene 15,15'-monooxygenase

Files

Original bundle

Collections