Study on the inhibitory activity of a synthetic defensin derived from barley endosperm against common food spoilage yeast
| dc.contributor.author | Shwaiki, Laila N. | |
| dc.contributor.author | Sahin, Aylin W. | |
| dc.contributor.author | Arendt, Elke K. | |
| dc.contributor.funder | Department of Agriculture, Food and the Marine, Ireland | en |
| dc.date.accessioned | 2022-03-03T16:05:56Z | |
| dc.date.available | 2022-03-03T16:05:56Z | |
| dc.date.issued | 2021-01 | |
| dc.date.updated | 2022-03-03T15:40:35Z | |
| dc.description.abstract | In the food industry, food spoilage is a real issue that can lead to a significant amount of waste. Although current preservation techniques are being applied to reduce the occurrence of spoilage microorganisms, the problem persists. Food spoilage yeast are part of this dilemma, with common spoilers such as Zygosaccharomyces, Kluyveromyces, Debaryomyces and Saccharomyces frequently encountered. Antimicrobial peptides derived from plants have risen in popularity due to their ability to reduce spoilage. This study examines the potential application of a synthetic defensin peptide derived from barley endosperm. Its inhibitory effect against common spoilage yeasts, its mechanisms of action (membrane permeabilisation and overproduction of reactive oxygen species), and its stability in different conditions were characterised. The safety of the peptide was evaluated through a haemolysis and cytotoxicity assay, and no adverse effects were found. Both assays were performed to understand the effect of the peptide if it were to be consumed. Its ability to be degraded by a digestive enzyme was also examined for its safety. Finally, the peptide was successfully applied to different beverages and maintained the same inhibitory effects in apple juice as was observed in the antiyeast assays, providing further support for its application in food preservation. | en |
| dc.description.sponsorship | Department of Agriculture, Food and the Marine, Ireland (Project Reference 15/F/731) | en |
| dc.description.status | Peer reviewed | en |
| dc.description.version | Published Version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.articleid | 165 | en |
| dc.identifier.citation | Shwaiki, L. N., Sahin, A. W. and Arendt, E. K. (2021) 'Study on the inhibitory activity of a synthetic defensin derived from barley endosperm against common food spoilage yeast', Molecules, 26 (1), 165 (14pp). doi: 10.3390/molecules26010165 | en |
| dc.identifier.doi | 10.3390/molecules26010165 | en |
| dc.identifier.endpage | 14 | en |
| dc.identifier.issn | 1420-3049 | |
| dc.identifier.issued | 1 | en |
| dc.identifier.journaltitle | Molecules | en |
| dc.identifier.startpage | 1 | en |
| dc.identifier.uri | https://hdl.handle.net/10468/12713 | |
| dc.identifier.volume | 26 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | Antimicrobial peptides | en |
| dc.subject | Antifungal activity | en |
| dc.subject | Disulfide bonds | en |
| dc.subject | Protein | en |
| dc.subject | Thionin | en |
| dc.subject | System | en |
| dc.title | Study on the inhibitory activity of a synthetic defensin derived from barley endosperm against common food spoilage yeast | en |
| dc.type | Article (peer-reviewed) | en |
