dc.contributor.author	Romano, Stefano
dc.contributor.author	Fernàndez-Guerra, Antonio
dc.contributor.author	Reen, F. Jerry
dc.contributor.author	Glöckner, Frank O.
dc.contributor.author	Crowley, Susan P.
dc.contributor.author	O'Sullivan, Orla
dc.contributor.author	Cotter, Paul D.
dc.contributor.author	Adams, Claire
dc.contributor.author	Dobson, Alan D. W.
dc.contributor.author	O'Gara, Fergal
dc.contributor.funder	European Commission
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	Department of Agriculture, Food and the Marine
dc.contributor.funder	Irish Research Council for Science, Engineering and Technology
dc.contributor.funder	Health Research Board
dc.contributor.funder	Irish Thoracic Society
dc.contributor.funder	Marine Institute
dc.contributor.funder	Teagasc
dc.date.accessioned	2017-06-20T11:39:46Z
dc.date.available	2017-06-20T11:39:46Z
dc.date.issued	2016-03-30
dc.description.abstract	Strains of the Pseudovibrio genus have been detected worldwide, mainly as part of bacterial communities associated with marine invertebrates, particularly sponges. This recurrent association has been considered as an indication of a symbiotic relationship between these microbes and their host. Until recently, the availability of only two genomes, belonging to closely related strains, has limited the knowledge on the genomic and physiological features of the genus to a single phylogenetic lineage. Here we present 10 newly sequenced genomes of Pseudovibrio strains isolated from marine sponges from the west coast of Ireland, and including the other two publicly available genomes we performed an extensive comparative genomic analysis. Homogeneity was apparent in terms of both the orthologous genes and the metabolic features shared amongst the 12 strains. At the genomic level, a key physiological difference observed amongst the isolates was the presence only in strain P. axinellae AD2 of genes encoding proteins involved in assimilatory nitrate reduction, which was then proved experimentally. We then focused on studying those systems known to be involved in the interactions with eukaryotic and prokaryotic cells. This analysis revealed that the genus harbors a large diversity of toxin-like proteins, secretion systems and their potential effectors. Their distribution in the genus was not always consistent with the phylogenetic relationship of the strains. Finally, our analyses identified new genomic islands encoding potential toxin-immunity systems, previously unknown in the genus. Our analyses shed new light on the Pseudovibrio genus, indicating a large diversity of both metabolic features and systems for interacting with the host. The diversity in both distribution and abundance of these systems amongst the strains underlines how metabolically and phylogenetically similar bacteria may use different strategies to interact with the host and find a niche within its microbiota. Our data suggest the presence of a sponge-specific lineage of Pseudovibrio. The reduction in genome size and the loss of some systems potentially used to successfully enter the host, leads to the hypothesis that P. axinellae strain AD2 may be a lineage that presents an ancient association with the host and that may be vertically transmitted to the progeny.	en
dc.description.sponsorship	Science Foundation Ireland (SSPC-2, 12/RC/2275; 13/TIDA/B2625; 12/TIDA/B2411; 12/TIDA/B2405; 14/TIDA/2438); Department of Agriculture, Food and the Marine (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council for Science, Engineering and Technology (PD/2011/2414;<s></s>GOIPG/2014/647); Health Research Board/Irish Thoracic Society (MRCG-2014-6); Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	387
dc.identifier.citation	Romano, S., Fernàndez-Guerra, A., Reen, F. J., Glöckner, F. O., Crowley, S. P., O'Sullivan, O., Cotter, P. D., Adams, C., Dobson, A. D. W. and O'Gara, F. (2016) 'Comparative genomic analysis reveals a diverse repertoire of genes involved in prokaryote-eukaryote interactions within the Pseudovibrio Genus', Frontiers in Microbiology, 7, 387 (20pp.). doi: 10.3389/fmicb.2016.00387	en
dc.identifier.doi	10.3389/fmicb.2016.00387
dc.identifier.endpage	20
dc.identifier.issn	1664-302X
dc.identifier.journaltitle	Frontiers in Microbiology	en
dc.identifier.startpage	1
dc.identifier.uri	https://hdl.handle.net/10468/4114
dc.identifier.volume	7
dc.language.iso	en	en
dc.publisher	Frontiers Media	en
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION
dc.relation.project	info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE
dc.relation.uri	http://journal.frontiersin.org/article/10.3389/fmicb.2016.00387/full
dc.rights	© 2016, Romano, Fernàndez-Guerra, Reen, Glöckner, Crowley, O'Sullivan, Cotter, Adams, Dobson and O'Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	Pseudovibrio	en
dc.subject	Comparative genomics	en
dc.subject	Symbiosis	en
dc.subject	Secretion systems	en
dc.subject	Toxins	en
dc.subject	Core-genome	en
dc.subject	Phylogenesis	en
dc.title	Comparative genomic analysis reveals a diverse repertoire of genes involved in prokaryote-eukaryote interactions within the Pseudovibrio Genus	en
dc.type	Article (peer-reviewed)	en

Comparative genomic analysis reveals a diverse repertoire of genes involved in prokaryote-eukaryote interactions within the Pseudovibrio Genus

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