Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure

Simple item page
dc.contributor.authorKelly, Sandra M.en
dc.contributor.authorLanigan, Noreenen
dc.contributor.authorO’Neill, Ian J.en
dc.contributor.authorBottacini, Francescaen
dc.contributor.authorLugli, Gabriele A.en
dc.contributor.authorViappiani, Aliceen
dc.contributor.authorTurroni, Francescaen
dc.contributor.authorVentura, Marcoen
dc.contributor.authorvan Sinderen, Douween
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderUniversità degli Studi di Parmaen
dc.date.accessioned2023-11-29T11:38:46Z
dc.date.available2023-11-29T11:38:46Z
dc.date.issued2020en
dc.description.abstractIn the current study, we show that biofilm formation by various strains and species belonging to Bifidobacterium, a genus that includes gut commensals with reported health-promoting activities, is induced by high concentrations of bile (0.5% (w/v) or higher) and individual bile salts (20 mM or higher), rather than by acid or osmotic stress. The transcriptomic response of a bifidobacterial prototype Bifidobacterium breve UCC2003 to such high bile concentrations was investigated and a random transposon bank of B. breve UCC2003 was screened for mutants that affect biofilm formation in order to identify genes involved in this adaptive process. Eleven mutants affected in their ability to form a biofilm were identified, while biofilm formation capacity of an insertional mutation in luxS and an exopolysaccharide (EPS) negative B. breve UCC2003 was also studied. Reduced capacity to form biofilm also caused reduced viability when exposed to porcine bile. We propose that bifidobacterial biofilm formation is an adaptive response to high concentrations of bile in order to avoid bactericidal effects of high bile concentrations in the gastrointestinal environment. Biofilm formation appears to be a multi-factorial process involving EPS production, proteins and extracellular DNA release, representing a crucial strategy in response to bile stress in order to enhance fitness in the gut environment.en
dc.description.sponsorshipUniversità degli Studi di Parma (HPC (High Performance Computing) facility)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid11598en
dc.identifier.citationKelly, S.M., Lanigan, N., O’Neill, I.J., Bottacini, F., Lugli, G.A., Viappiani, A., Turroni, F., Ventura, M. and Van Sinderen, D. (2020) ‘Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure’, Scientific Reports, 10(1), 11598 (14pp). doi: 10.1038/s41598-020-68179-9en
dc.identifier.doi10.1038/s41598-020-68179-9en
dc.identifier.endpage14en
dc.identifier.issn2045-2322en
dc.identifier.issued1en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/15273
dc.identifier.volume10en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.ispartofScientific Reportsen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/en
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacterial physiologyen
dc.subjectBacteriologyen
dc.subjectBiofilmsen
dc.subjectMicrobial ecologyen
dc.subjectMicrobiologyen
dc.subjectMicrobiomeen
dc.titleBifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposureen
dc.typeArticle (peer-reviewed)en
dc.typejournal-articleen
oaire.citation.issue1en
oaire.citation.volume10en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
s41598-020-68179-9.pdf
Size:
3.11 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Thumbnail Image
Name:
41598_2020_68179_MOESM1_ESM.pdf
Size:
94.19 KB
Format:
Adobe Portable Document Format
Description:
Supplementary Information
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles