Further elucidation of galactose utilization in Lactococcus lactis MG1363

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dc.contributor.authorSolopova, Ana
dc.contributor.authorBachman, Herwig
dc.contributor.authorTeusink, Bas
dc.contributor.authorKok, Jan
dc.contributor.authorKuipers, Oscar P.
dc.contributor.funderNederlandse Organisatie voor Wetenschappelijk Onderzoek
dc.contributor.funderStichting voor de Technische Wetenschappen
dc.date.accessioned2018-08-29T15:47:18Z
dc.date.available2018-08-29T15:47:18Z
dc.date.issued2018
dc.description.abstractSince the 1970s, galactose metabolism in Lactococcus lactis has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP-dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some Lactococcus strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTSGal exists. However, the PTSGal transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in L. lactis MG1363.en
dc.description.sponsorshipNederlandse Organisatie voor Wetenschappelijk Onderzoek - Stichting voor de Technische Wetenschappen (10619)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid1803
dc.identifier.citationSolopova, A., Bachmann, H., Teusink, B., Kok, J. and Kuipers, O. P. (2018) 'Further elucidation of galactose utilization in Lactococcus lactis MG1363', Frontiers in Microbiology, 9, 1803 (9pp). doi: 10.3389/fmicb.2018.01803en
dc.identifier.doi10.3389/fmicb.2018.01803
dc.identifier.endpage9
dc.identifier.issn1664-302X
dc.identifier.journaltitleFrontiers in Microbiologyen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/6656
dc.identifier.volume9
dc.language.isoenen
dc.publisherFrontiers Media S.A.en
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.01803/full
dc.rights© 2018, the Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectGalactose transporteren
dc.subjectPTSen
dc.subjectPEP-transferase systemen
dc.subjectLactococcus lactisen
dc.subjectGalPen
dc.titleFurther elucidation of galactose utilization in Lactococcus lactis MG1363en
dc.typeArticle (peer-reviewed)en
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