Antibiotic susceptibility profiles of dairy Leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix

Simple item page
dc.contributor.authorFlórez, Ana Belén
dc.contributor.authorCampedelli, Ilenia
dc.contributor.authorDelgado, Susana
dc.contributor.authorAlegría, Ángel
dc.contributor.authorSalvetti, Elisa
dc.contributor.authorFelis, Giovanna E.
dc.contributor.authorMayo, Baltasar
dc.contributor.authorTorriani, Sandra
dc.date.accessioned2017-02-27T12:45:52Z
dc.date.available2017-02-27T12:45:52Z
dc.date.issued2016-01-04
dc.description.abstractIn spite of a global concern on the transfer of antibiotic resistances (AR) via the food chain, limited information exists on this issue in species of Leuconostoc and Weissella, adjunct cultures used as aroma producers in fermented foods. In this work, the minimum inhibitory concentration was determined for 16 antibiotics in 34 strains of dairy origin, belonging to Leuconostoc mesenteroides (18), Leuconostoc citreum (11), Leuconostoc lactis (2), Weissella hellenica (2), and Leuconostoc carnosum (1). Atypical resistances were found for kanamycin (17 strains), tetracycline and chloramphenicol (two strains each), and erythromycin, clindamycin, virginiamycin, ciprofloxacin, and rifampicin (one strain each). Surprisingly, L. mesenteroides subsp. mesenteroides LbE16, showed resistance to four antibiotics, kanamycin, streptomycin, tetracycline and virginiamycin. PCR analysis identified tet(S) as responsible for tetracycline resistance in LbE16, but no gene was detected in a second tetracycline-resistant strain, L. mesenteroides subsp. cremoris LbT16. In Leuconostoc mesenteroides subsp. dextranicum LbE15, erythromycin and clindamycin resistant, an erm(B) gene was amplified. Hybridization experiments proved erm(B) and tet(S) to be associated to a plasmid of ≈35 kbp and to the chromosome of LbE15 and LbE16, respectively. The complete genome sequence of LbE15 and LbE16 was used to get further insights on the makeup and genetic organization of AR genes. Genome analysis confirmed the presence and location of erm(B) and tet(S), but genes providing tetracycline resistance in LbT16 were again not identified. In the genome of the multi-resistant strain LbE16, genes that might be involved in aminoglycoside (aadE, aphA-3, sat4) and virginiamycin [vat(E)] resistance were further found. The erm(B) gene but not tet(S) was transferred from Leuconostoc to Enterococcus faecalis both under laboratory conditions and in cheese. This study contributes to the characterization of AR in the Leuconostoc-Weissella group, provides evidence of the genetic basis of atypical resistances, and demonstrates the inter-species transfer of erythromycin resistance.en
dc.description.sponsorshipSpain-Italy bilateral collaboration program (Ref. Ministerio de Educatiòn y Ciencia IT2009-0080, and Ministerio dell'Istruzione, dell'Univesità e della Ricerca IT105MD12Len
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationFlórez, A. B., Campedelli, I., Delgado, S., Alegría, Á., Salvetti, E., Felis, G. E., Mayo, B. and Torriani, S. (2016) 'Antibiotic Susceptibility Profiles of Dairy Leuconostoc, Analysis of the Genetic Basis of Atypical Resistances and Transfer of Genes In Vitro and in a Food Matrix', PLOS ONE, 11(1), pp. e0145203. doi:10.1371/journal.pone.0145203en
dc.identifier.doi10.1371/journal.pone.0145203
dc.identifier.endpagee0145203-20en
dc.identifier.issn1932-6203
dc.identifier.journaltitlePLoS ONEen
dc.identifier.startpagee0145203-1en
dc.identifier.urihttps://hdl.handle.net/10468/3697
dc.identifier.volume11en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2016 Flórez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectAntibiotic resistanceen
dc.subjectAntibioticsen
dc.subjectAntimicrobial resistanceen
dc.subjectEnterococcus faecalisen
dc.subjectPolymerase chain reactionen
dc.subjectErythromycinen
dc.subjectTetracyclinesen
dc.subjectChloramphenicolen
dc.titleAntibiotic susceptibility profiles of dairy Leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrixen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
2577.PDF
Size:
1001.66 KB
Format:
Adobe Portable Document Format
Description:
Published Version
Download
Thumbnail Image
Name:
2577_1.docx
Size:
77.56 KB
Format:
Microsoft Word XML
Description:
Supplementary Information
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles