Loss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis.

Simple item page
dc.contributor.authorJiricny, Natalie
dc.contributor.authorMolin, Soren
dc.contributor.authorFoster, Kevin
dc.contributor.authorDiggle, Stephen P.
dc.contributor.authorScanlan, Pauline D.
dc.contributor.authorGhoul, Melanie
dc.contributor.authorJohansen, Helle Krogh
dc.contributor.authorSantorelli, Lorenzo A.
dc.contributor.authorPopat, Roman
dc.contributor.authorWest, Stuart A.
dc.contributor.authorGriffin, Ashleigh S.
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen
dc.contributor.funderRoyal Societyen
dc.contributor.funderL'Oreal USAen
dc.contributor.funderUnited Nations Educational, Scientific and Cultural Organizationen
dc.contributor.funderNatural Environment Research Councilen
dc.date.accessioned2018-07-02T14:54:54Z
dc.date.available2018-07-02T14:54:54Z
dc.date.issued2014-01-14
dc.date.updated2018-07-01T21:20:00Z
dc.description.abstractPseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.en
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BBSRC studentship); Royal Society, L'Oreal USA and United Nations Educational, Scientific and Cultural Organization (Royal Society and L'Oreal/UNESCO Fellowship for Women in Science); Natural Environment Research Council (NERC Grant Ref: NE/J007064)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJiricny, N., Molin, S., Foster, K., Diggle, S. P., Scanlan, P. D., Ghoul, M., Johansen, H. K., Santorelli, L. A., Popat, R., West, S. A. and Griffin, A. S. (2014) 'Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis', PLOS ONE, 9(1), pp. e83124. doi: 10.1371/journal.pone.0083124en
dc.identifier.doi10.1371/journal.pone.0083124
dc.identifier.endpagee83124-10en
dc.identifier.issn1932-6203
dc.identifier.issued1en
dc.identifier.journaltitlePlos Oneen
dc.identifier.startpagee83124-1en
dc.identifier.urihttps://hdl.handle.net/10468/6402
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2014 Jiricny et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiofilmsen
dc.subjectCell communicationen
dc.subjectCystic fibrosisen
dc.subjectExtracellular spaceen
dc.subjectPancreatic elastaseen
dc.subjectPeptide hydrolasesen
dc.subjectPseudomonas aeruginosaen
dc.subjectSiderophoresen
dc.subjectBacterial enzymeen
dc.subjectLungen
dc.subjectBacterial geneen
dc.subjectBacterial metabolismen
dc.subjectBacterial secretion systemen
dc.subjectBacterial strainen
dc.titleLoss of social behaviours in populations of Pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis.en
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
journal.pone.0083124.PDF
Size:
689 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download
Thumbnail Image
Name:
6030_File_S1.docx
Size:
61.04 KB
Format:
Microsoft Word XML
Description:
Supporting Information
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles