Adult microbiota-deficient mice have distinct dendritic morphological changes: Differential effects in the amygdala and hippocampus

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dc.contributor.authorLuczynski, Pauline
dc.contributor.authorWhelan, Seán O.
dc.contributor.authorO'Sullivan, Colette
dc.contributor.authorClarke, Gerard
dc.contributor.authorShanahan, Fergus
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorCryan, John F.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderGlaxoSmithKlineen
dc.contributor.funderPfizeren
dc.contributor.funderWyethen
dc.contributor.funderMead Johnson Nutritionen
dc.contributor.funderNational Alliance for Research on Schizophrenia and Depressionen
dc.contributor.funderBrain and Behavior Research Foundationen
dc.contributor.funderIrish Government's National Development Planen
dc.date.accessioned2019-11-04T10:33:02Z
dc.date.available2019-11-04T10:33:02Z
dc.date.issued2016-10-31
dc.description.abstractIncreasing evidence implicates the microbiota in the regulation of brain and behaviour. Germ‐free mice (GF; microbiota deficient from birth) exhibit altered stress hormone signalling and anxiety‐like behaviours as well as deficits in social cognition. Although the mechanisms underlying the ability of the gut microbiota to influence stress responsivity and behaviour remain unknown, many lines of evidence point to the amygdala and hippocampus as likely targets. Thus, the aim of this study was to determine if the volume and dendritic morphology of the amygdala and hippocampus differ in GF versus conventionally colonized (CC) mice. Volumetric estimates revealed significant amygdalar and hippocampal expansion in GF compared to CC mice. We also studied the effect of GF status on the level of single neurons in the basolateral amygdala (BLA) and ventral hippocampus. In the BLA, the aspiny interneurons and pyramidal neurons of GF mice exhibited dendritic hypertrophy. The BLA pyramidal neurons of GF mice had more thin, stubby and mushroom spines. In contrast, the ventral hippocampal pyramidal neurons of GF mice were shorter, less branched and had less stubby and mushroom spines. When compared to controls, dentate granule cells of GF mice were less branched but did not differ in spine density. These findings suggest that the microbiota is required for the normal gross morphology and ultrastructure of the amygdala and hippocampus and that this neural remodelling may contribute to the maladaptive stress responsivity and behavioural profile observed in GF mice.en
dc.description.sponsorshipHealth Research Awards (Grant Numbers HRA_POR/2012/32, HRA_POR/2014/647); Brain and Behavior Research Foundation (Grant Number: 20771)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLuczynski, P., Whelan, S. O., O'Sullivan, C., Clarke, G., Shanahan, F., Dinan, T. G. and Cryan, J. F. (2016) 'Adult microbiota-deficient mice have distinct dendritic morphological changes: differential effects in the amygdala and hippocampus', European Journal of Neuroscience, 44(9), pp. 2654-2666. (13pp.) DOI: 10.1111/ejn.13291en
dc.identifier.doi10.1111/ejn.13291en
dc.identifier.eissn1460-9568
dc.identifier.endpage2666en
dc.identifier.issn0953-816X
dc.identifier.issued9en
dc.identifier.journaltitleEuropean Journal of Neuroscienceen
dc.identifier.startpage2654en
dc.identifier.urihttps://hdl.handle.net/10468/8946
dc.identifier.volume44en
dc.language.isoenen
dc.publisherWileyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/613979/EU/Microbiome Influence on Energy balance and Brain Development-Function Put into Action to Tackle Diet-related Diseases and Behavior./MYNEWGUTen
dc.relation.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/ejn.13291
dc.rights©2016 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBrain volumeen
dc.subjectDendritic spinesen
dc.subjectDesign-based stereologyen
dc.subjectGerm-free mouseen
dc.subjectMicrobiota-gut-brain axisen
dc.titleAdult microbiota-deficient mice have distinct dendritic morphological changes: Differential effects in the amygdala and hippocampusen
dc.typeArticle (peer-reviewed)en
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