Human milk oligosaccharide-sharing by a consortium of infant derived Bifidobacterium species

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Date
2022-03
Authors
Walsh, Clodagh
Lane, Jonathan A.
van Sinderen, Douwe
Hickey, Rita M.
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Nature Research
Published Version
10.1038/s41598-022-07904-y
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Abstract
Bifidobacteria are associated with a host of health benefits and are typically dominant in the gut microbiota of healthy, breast-fed infants. A key adaptation, facilitating the establishment of these species, is their ability to consume particular sugars, known as human milk oligosaccharides (HMO), which are abundantly found in breastmilk. In the current study, we aimed to characterise the co-operative metabolism of four commercial infant-derived bifidobacteria (Bifidobacterium bifidum R0071, Bifidobacterium breve M-16V, Bifidobacterium infantis R0033, and Bifidobacterium infantis M-63) when grown on HMO. Three different HMO substrates (2 '-fucosyllactose alone and oligosaccharides isolated from human milk representing non-secretor and secretor status) were employed. The four-strain combination resulted in increased bifidobacterial numbers (> 21%) in comparison to single strain cultivation. The relative abundance of B. breve increased by > 30% during co-cultivation with the other strains despite demonstrating limited ability to assimilate HMO in mono-culture. HPLC analysis revealed strain-level variations in HMO consumption. Metabolomics confirmed the production of formate, acetate, 1,2-propanediol, and lactate with an overall increase in such metabolites during co-cultivation. These results support the concept of positive co-operation between multiple bifidobacterial strains during HMO utilisation which may result in higher cell numbers and a potentially healthier balance of metabolites.
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Keywords
Chain fatty-acids , Enteropathogenic infection , Gut microbiota , Double-blind , Cow milk , Consumption , Reveals , Metabolism , Probiotics , Strains
Citation
Walsh, C., Lane, J.A., Van Sinderen, D. and Hickey, R.M. (2022) ‘Human milk oligosaccharide-sharing by a consortium of infant derived Bifidobacterium species’, Scientific Reports, 12(1) 4143 (14pp). doi: 10.1038/s41598-022-07904-y
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https://hdl.handle.net/10468/14450
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APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles
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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.