Metagenomics of Atacama lithobiontic extremophile life unveils highlights on fungal communities, biogeochemical cycles and carbohydrate-active enzymes

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dc.contributor.authorGómez-Silva, Benito
dc.contributor.authorVilo-Muñoz, Claudia
dc.contributor.authorGaletović, Alexandra
dc.contributor.authorDong, Qunfeng
dc.contributor.authorCastelán-Sánchez, Hugo G.
dc.contributor.authorPérez-Llano, Yordanis
dc.contributor.authorSánchez-Carbente, María del Rayo
dc.contributor.authorDávila-Ramos, Sonia
dc.contributor.authorCortés-López, Nohemí Gabriela
dc.contributor.authorMartínez-Ávila, Liliana
dc.contributor.authorDobson, Alan D. W.
dc.contributor.authorBatista-García, Ramón Alberto
dc.contributor.funderUniversidad de Antofagastaen
dc.contributor.funderUniversity of Texas Health Science Center at San Antonioen
dc.contributor.funderNational Institutes of Healthen
dc.contributor.funderCancer Prevention and Research Institute of Texasen
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderConsejo Nacional de Ciencia y Tecnologíaen
dc.contributor.funderComisión Nacional de Investigación Científica y Tecnológicaen
dc.contributor.funderCentre for Biotechnology & Bioinformaticsen
dc.date.accessioned2019-12-10T09:20:44Z
dc.date.available2019-12-10T09:20:44Z
dc.date.issued2019-11-27
dc.description.abstractHalites, which are typically found in various Atacama locations, are evaporitic rocks that are considered as micro-scaled salterns. Both structural and functional metagenomic analyses of halite nodules were performed. Structural analyses indicated that the halite microbiota is mainly composed of NaCl-adapted microorganisms. In addition, halites appear to harbor a limited diversity of fungal families together with a biodiverse collection of protozoa. Functional analysis indicated that the halite microbiome possesses the capacity to make an extensive contribution to carbon, nitrogen, and sulfur cycles, but possess a limited capacity to fix nitrogen. The halite metagenome also contains a vast repertory of carbohydrate active enzymes (CAZY) with glycosyl transferases being the most abundant class present, followed by glycosyl hydrolases (GH). Amylases were also present in high abundance, with GH also being identified. Thus, the halite microbiota is a potential useful source of novel enzymes that could have biotechnological applicability. This is the first metagenomic report of fungi and protozoa as endolithobionts of halite nodules, as well as the first attempt to describe the repertoire of CAZY in this community. In addition, we present a comprehensive functional metagenomic analysis of the metabolic capacities of the halite microbiota, providing evidence for the first time on the sulfur cycle in Atacama halites.en
dc.description.sponsorshipCeBiB (FB-0001); Universidad de Antofagasta (SI-5305); UT Health San Antonio (Project Number NIH-NCI P30 CA054174); CPRIT Core Facility (Award Number RP160732); Irish Department of Agriculture, Food and the Marine (DAFM) (SMI-BIO project (15/F/698)); CONACyT-Mexico (Projects No. 285816)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid619en
dc.identifier.citationGómez-Silva, B., Vilo-Muñoz, C., Galetović, A., Dong, Q., Castelán-Sánchez, H. G., Pérez-Llano, Y., Sánchez-Carbente, M. d. R., Dávila-Ramos, S., Cortés-López, N. G., Martínez-Ávila, L., Dobson, A. D. W. and Batista-García, R. A. (2019) 'Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes', Microorganisms, 7(12), 619 (25pp). doi: 10.3390/microorganisms7120619en
dc.identifier.doi10.3390/microorganisms7120619en
dc.identifier.eissn2076-2607
dc.identifier.endpage25en
dc.identifier.issued12en
dc.identifier.journaltitleMicroorganismsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9368
dc.identifier.volume7en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/299091/EU/How do groundwater-surface water interactions control recharge from ephemeral streams?/EPHEMERAL GSIen
dc.relation.projectinfo:eu-repo/grantAgreement/NIH/OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH/1S10OD021805-01/US/High Throughput DNA Sequencer: Illumina HiSeq 3000 Sequencer/en
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAtacame Deserten
dc.subjectHaliteen
dc.subjectFunctional metagenomicsen
dc.subjectEndolithic fungien
dc.subjectCAZymeen
dc.titleMetagenomics of Atacama lithobiontic extremophile life unveils highlights on fungal communities, biogeochemical cycles and carbohydrate-active enzymesen
dc.typeArticle (peer-reviewed)en
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