Social interaction-induced activation of RNA splicing in the amygdala of microbiome-deficient mice

Stilling, Roman M.; Moloney, Gerard M.; Ryan, Feargal J.; Hoban, Alan E.; Bastiaanssen, Thomaz F. S.; Shanahan, Fergus; Clarke, Gerard; Claesson, Marcus J.; Dinan, Timothy G.; Cryan, John F.

Social interaction-induced activation of RNA splicing in the amygdala of microbiome-deficient mice

dc.contributor.author	Stilling, Roman M.
dc.contributor.author	Moloney, Gerard M.
dc.contributor.author	Ryan, Feargal J.
dc.contributor.author	Hoban, Alan E.
dc.contributor.author	Bastiaanssen, Thomaz F. S.
dc.contributor.author	Shanahan, Fergus
dc.contributor.author	Clarke, Gerard
dc.contributor.author	Claesson, Marcus J.
dc.contributor.author	Dinan, Timothy G.
dc.contributor.author	Cryan, John F.
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	Irish Research Council	en
dc.contributor.funder	Irish Health Board	en
dc.contributor.funder	National Alliance for Research on Schizophrenia and Depression	en
dc.date.accessioned	2019-10-29T05:35:14Z
dc.date.available	2019-10-29T05:35:14Z
dc.date.issued	2018-05-29
dc.description.abstract	Social behaviour is regulated by activity of host-associated microbiota across multiple species. However, the molecular mechanisms mediating this relationship remain elusive. We therefore determined the dynamic, stimulus-dependent transcriptional regulation of germ-free (GF) and GF mice colonised post weaning (exGF) in the amygdala, a brain region critically involved in regulating social interaction. In GF mice the dynamic response seen in controls was attenuated and replaced by a marked increase in expression of splicing factors and alternative exon usage in GF mice upon stimulation, which was even more pronounced in exGF mice. In conclusion, we demonstrate a molecular basis for how the host microbiome is crucial for a normal behavioural response during social interaction. Our data further suggest that social behaviour is correlated with the gene-expression response in the amygdala, established during neurodevelopment as a result of host-microbe interactions. Our findings may help toward understanding neurodevelopmental events leading to social behaviour dysregulation, such as those found in autism spectrum disorders (ASDs).	en
dc.description.sponsorship	Irish Research Council (GOIPD/2014/355); National Alliance for Research on Schizophrenia and Depression (20771)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	e33070	en
dc.identifier.citation	Stilling, R. M., Moloney, G. M., Ryan, F. J., Hoban, A. E., Bastiaanssen, T. F. S., Shanahan, F., Clarke, G., Claesson, M. J., Dinan, T. G. and Cryan, J. F., 2018. Social interaction-induced activation of RNA splicing in the amygdala of microbiome-deficient mice. Elife, 7, e33070, (21 pp.). DOI: 10.7554/eLife.33070	en
dc.identifier.doi	10.7554/eLife.33070	en
dc.identifier.eissn	2050-084X
dc.identifier.endpage	21	en
dc.identifier.journaltitle	eLife	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/8903
dc.identifier.volume	7	en
dc.language.iso	en	en
dc.publisher	eLife Sciences Publications Ltd	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/	en
dc.relation.uri	https://elifesciences.org/articles/33070
dc.rights	© Copyright Stilling et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	en
dc.subject	Social behaviour	en
dc.subject	Neurodevelopment	en
dc.subject	Microbiome	en
dc.subject	Amygdala	en
dc.subject	Activity-dependent transcription	en
dc.subject	Autism	en
dc.title	Social interaction-induced activation of RNA splicing in the amygdala of microbiome-deficient mice	en
dc.type	Article (peer-reviewed)	en