RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection

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dc.contributor.author Konczal, Mateusz
dc.contributor.author Ellison, Amy R.
dc.contributor.author Phillips, Karl P.
dc.contributor.author Radwan, Jacek
dc.contributor.author Mohammed, Ryan S.
dc.contributor.author Cable, Joanne
dc.contributor.author Chadzinska, Magdalena
dc.date.accessioned 2020-09-17T08:52:16Z
dc.date.available 2020-09-17T08:52:16Z
dc.date.issued 2020-08-01
dc.identifier.citation Konczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology. doi: 10.1111/pim.12782 en
dc.identifier.issn 0141-9838
dc.identifier.uri http://hdl.handle.net/10468/10535
dc.identifier.doi 10.1111/pim.12782 en
dc.description.abstract Gyrodactylids are ubiquitous ectoparasites of teleost fish, but our understanding of the host immune response against them is fragmentary. Here, we used RNA-Seq to investigate genes involved in the primary response to infection with Gyrodactylus bullatarudis on the skin of guppies, Poecilia reticulata, an important evolutionary model, but also one of the most common fish in the global ornamental trade. Analysis of differentially expressed genes identified several immune-related categories, including IL-17 signalling pathway and Th17 cell differentiation, cytokine-cytokine receptor interaction, chemokine signalling pathway, NOD-like receptor signalling pathway, natural killer cell-mediated cytotoxicity and pathways involved in antigen recognition, processing and presentation. Components of both the innate and the adaptive immune responses play a role in response to gyrodactylid infection. Genes involved in IL-17/Th17 response were particularly enriched among differentially expressed genes, suggesting a significant role for this pathway in fish responses to ectoparasites. Our results revealed a sizable list of genes potentially involved in the teleost-gyrodactylid immune response. en
dc.description.sponsorship Narodowe Centrum Nauki (Grant Numbers: UMO‐2013/10/M/NZ8/00253 , UMO‐2016/20/S/NZ8/00208); Poznan RNA Research Centre, Poland (Grant Number: 01/KNOW2/2014); Biotechnology and Biological Sciences Research Council (Future Leader Fellowship Grant Number: BB/R010609/1) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Ltd. en
dc.rights © 2020, John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Konczal, M., Ellison, A. R., Phillips, K. P., Radwan, J., Mohammed, R. S., Cable, J. and Chadzinska, M. (2020) 'RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection', Parasite Immunology, doi: 10.1111/pim.12782, which has been published in final form at https://doi.org/10.1111/pim.12782. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. en
dc.subject Ectoparasites en
dc.subject Fish en
dc.subject Guppy en
dc.subject Gyrodactylus en
dc.subject Immunity en
dc.subject RNA-Seq en
dc.subject Th17 response en
dc.subject Transcriptome en
dc.title RNA-Seq analysis of the guppy immune response against Gyrodactylus bullatarudis infection en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Karl Phillips, Zoology & Ecology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: karl.phillips@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2021-08-01
dc.date.updated 2020-09-16T12:13:59Z
dc.description.version Accepted Version en
dc.internal.rssid 536050914
dc.contributor.funder Narodowe Centrum Nauki en
dc.contributor.funder Poznan RNA Research Centre, Poland en
dc.contributor.funder Llywodraeth Cymru en
dc.contributor.funder Higher Education Funding Council for Wales en
dc.contributor.funder Biotechnology and Biological Sciences Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Parasite Immunology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress karl.phillips@ucc.ie en
dc.identifier.articleid e12782 en
dc.internal.bibliocheck In press. Check vol / issue / page range. Amend citation and copyright statement as necessary. en
dc.identifier.eissn 1365-3024


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