Devil's Claw to suppress appetite - ghrelin receptor modulation potential of a Harpagophytum procumbens root extract

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dc.contributor.author Torres-Fuentes, Cristina
dc.contributor.author Theeuwes, Wessel F.
dc.contributor.author McMullen, Michael K.
dc.contributor.author McMullen, Anna K.
dc.contributor.author Dinan, Timothy G.
dc.contributor.author Cryan, John F.
dc.contributor.author Schellekens, Harriët
dc.date.accessioned 2016-02-17T11:43:39Z
dc.date.available 2016-02-17T11:43:39Z
dc.date.issued 2014
dc.identifier.citation Torres-Fuentes C, Theeuwes WF, McMullen MK, McMullen AK, Dinan TG, Cryan JF, et al. (2014) Devil's Claw to Suppress Appetite—Ghrelin Receptor Modulation Potential of a Harpagophytum procumbens Root Extract. PLoS ONE 9(7): e103118. doi:10.1371/journal.pone.0103118
dc.identifier.volume 9 en
dc.identifier.issued 7 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2329
dc.identifier.doi 10.1371/journal.pone.0103118
dc.description.abstract Ghrelin is a stomach-derived peptide that has been identified as the only circulating hunger hormone that exerts a potent orexigenic effect via activation of its receptor, the growth hormone secretagogue receptor (GHS-R1a). Hence, the ghrelinergic system represents a promising target to treat obesity and obesity-related diseases. In this study we analysed the GHS-R1a receptor activating potential of Harpagophytum procumbens, popularly known as Devil's Claw, and its effect on food intake in vivo. H. procumbens is an important traditional medicinal plant from Southern Africa with potent anti-inflammatory and analgesic effects. This plant has been also used as an appetite modulator but most evidences are anecdotal and to our knowledge, no clear scientific studies relating to appetite modulation have been done to this date. The ghrelin receptor activation potential of an extract derived from the dried tuberous roots of H. procumbens was analysed by calcium mobilization and receptor internalization assays in human embryonic kidney cells (Hek) stably expressing the GHS-R1a receptor. Food intake was investigated in male C57BL/6 mice following intraperitoneal administration of H. procumbens root extract in ad libitum and food restricted conditions. Exposure to H. procumbens extract demonstrated a significant increased cellular calcium influx but did not induce subsequent GHS-R1a receptor internalization, which is a characteristic for full receptor activation. A significant anorexigenic effect was observed in male C57BL/6 mice following peripheral administration of H. procumbens extract. We conclude that H. procumbens root extract is a potential novel source for potent anti-obesity bioactives. These results reinforce the promising potential of natural bioactives to be developed into functional foods with weight-loss and weight maintenance benefits. en
dc.description.sponsorship Enterprise Ireland (Grant No. CC20080001); Science Foundation Ireland (SFI Grant No. 02/CE/B124; 07/CE/B1368; Centre Grant to Alimentary Pharmabiotic Centre, University College Cork) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 Torres-Fuentes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Dietary fiber en
dc.subject Hormone en
dc.subject Desensitization en
dc.subject Harpagoside en
dc.subject Arrestins en
dc.subject Peptides en
dc.subject Proteins en
dc.subject Stomach en
dc.subject Agonist en
dc.subject Obesity en
dc.title Devil's Claw to suppress appetite - ghrelin receptor modulation potential of a Harpagophytum procumbens root extract en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Harriet Schellekens, Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland. +353-21-490-3000 Email: h.schellekens@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000339993700036
dc.contributor.funder Enterprise Ireland
dc.contributor.funder Science Foundation Ireland
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress h.schellekens@ucc.ie en
dc.identifier.articleid e103118


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© 2015 Torres-Fuentes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 Torres-Fuentes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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