Exposure of Bifidobacterium longum subsp infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response

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dc.contributor.author Kavanaugh, Devon W.
dc.contributor.author O'Callaghan, John
dc.contributor.author Butto, Ludovica F.
dc.contributor.author Slattery, Helen
dc.contributor.author Lane, Jonathan
dc.contributor.author Clyne, Marguerite
dc.contributor.author Kane, Marian
dc.contributor.author Joshi, Lokesh
dc.contributor.author Hickey, Rita M.
dc.date.accessioned 2016-02-17T11:45:33Z
dc.date.available 2016-02-17T11:45:33Z
dc.date.issued 2013
dc.identifier.citation Kavanaugh DW, O’Callaghan J, Buttó LF, Slattery H, Lane J, Clyne M, et al. (2013) Exposure of Bifidobacterium longum subsp. infantis to Milk Oligosaccharides Increases Adhesion to Epithelial Cells and Induces a Substantial Transcriptional Response. PLoS ONE 8(6): e67224. doi:10.1371/journal.pone.0067224 en
dc.identifier.volume 8 en
dc.identifier.issued 6 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2373
dc.identifier.doi 10.1371/journal.pone.0067224
dc.description.abstract In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (3'sialyllactose and 6'sialyllactose) and a commercial prebiotic (Beneo Orafti P95; oligofructose) were assayed for their ability to promote adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 and Caco-2 human intestinal cells. Treatment with the commercial prebiotic or 3'sialyllactose did not enhance adhesion. However, treatment with 6'sialyllactose resulted in increased adhesion (4.7 fold), while treatment with a mixture of 3'- and 6'-sialyllactose substantially increased adhesion (9.8 fold) to HT-29 intestinal cells. Microarray analyses were subsequently employed to investigate the transcriptional response of B. longum subsp. infantis to the different oligosaccharide treatments. This data correlated strongly with the observed changes in adhesion to HT-29 cells. The combination of 3'- and 6'-sialyllactose resulted in the greatest response at the genetic level (both in diversity and magnitude) followed by 6'sialyllactose, and 3'sialyllactose alone. The microarray data was further validated by means of real-time PCR. The current findings suggest that the increased adherence phenotype of Bifidobacterium longum subsp. infantis resulting from exposure to milk oligosaccharides is multi-faceted, involving transcription factors, chaperone proteins, adhesion-related proteins, and a glycoside hydrolase. This study gives additional insight into the role of milk oligosaccharides within the human intestine and the molecular mechanisms underpinning host-microbe interactions. en
dc.description.sponsorship Science Foundation Ireland (SFI Grant No. 08/SRC/B1393, Alimentary Glycoscience Research Cluster (AGRC)); Teagasc (Walsh Fellowship). en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2013 Kavanaugh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Heat shock protein en
dc.subject Bottle fed infants en
dc.subject In-vitro en
dc.subject Helicobacter pylori en
dc.subject Caco-2 cells en
dc.subject Intestinal microflora en
dc.subject Haemophilus ducreyi en
dc.subject Bacterial adhesion en
dc.subject Human enterocyte en
dc.subject Strains en
dc.title Exposure of Bifidobacterium longum subsp infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Ludovica F. Butto, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000320846500130
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress rita.hickey@teagasc.ie en
dc.identifier.articleid e67224


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© 2013 Kavanaugh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2013 Kavanaugh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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