Novel N4-like bacteriophages of pectobacterium atrosepticum

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dc.contributor.author Buttimer, Colin
dc.contributor.author Hendrix, Hanne
dc.contributor.author Lucid, Alan
dc.contributor.author Neve, Horst
dc.contributor.author Noben, Jean-Paul
dc.contributor.author Franz, Charles M. A. P.
dc.contributor.author O'Mahony, Jim
dc.contributor.author Lavigne, Rob
dc.contributor.author Coffey, Aidan
dc.date.accessioned 2018-09-27T12:08:10Z
dc.date.available 2018-09-27T12:08:10Z
dc.date.issued 2018
dc.identifier.citation Buttimer, C., Hendrix, H., Lucid, A., Neve, H., Noben, J.-P., Franz, C., O’Mahony, J., Lavigne, R. and Coffey, A. (2018) 'Novel N4-like bacteriophages of pectobacterium atrosepticum', Pharmaceuticals, 11(2), 45 (22pp). doi: 10.3390/ph11020045 en
dc.identifier.volume 11
dc.identifier.issued 2
dc.identifier.startpage 1
dc.identifier.endpage 22
dc.identifier.issn 1424-8247
dc.identifier.uri http://hdl.handle.net/10468/6919
dc.identifier.doi 10.3390/ph11020045
dc.description.abstract Pectobacterium atrosepticum is an economically important phytopathogen that is responsible for potato blackleg and soft rot, and for which current control strategies are limited. In this study, stem samples of potato crops exhibiting blackleg were taken from three farms in Co. Cork, Ireland, and they were found to be infected with P. atrosepticum. Three closely related bacteriophages (phages) that are specific to this phytopathogen were isolated and characterized, namely vB_PatP_CB1, vB_PatP_CB3, and vB_PatP_CB4 (abbreviated as CB1, CB3, and CB4). Both CB1 and CB3 were determined to infect 12 strains and CB4 10 strains of the 19 strains of P. atrosepticum tested. Morphology, latent periods, burst sizes, and their stability at various temperatures and pHs were also examined. Genome sequencing of the three phages revealed that they shared a minimum nucleotide identity of 93% with each other. Their genomes exhibited an Enquartavirinae genome organization, possessing several conserved proteins that were associated with phages of this group, like the type species Escherichia virus N4. Tandem electrospray ionization-mass spectrometry (ESI-MS/MS) allowed for the identification of ten structural proteins that form the virion of CB1, six that are conserved in phage N4. Biocontrol experiments demonstrated that the phages suppress soft rot formation upon co-inoculation with P. atrosepticum on whole tubers. The results of this study indicate that CB1 related phages could be good candidates for phage-based control. en
dc.description.sponsorship KU Leuven (GOA project ‘Phage Biosystems' ) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI AG en
dc.relation.uri http://www.mdpi.com/1424-8247/11/2/45
dc.rights © 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject Phage isolation en
dc.subject Phage characterization en
dc.subject Pectobacterium en
dc.subject Blackleg en
dc.subject Soft rot en
dc.subject Phage-mediated biocontrol of bacteria en
dc.subject Plant disease biocontrol en
dc.subject Phage therapy en
dc.subject N4-like phage en
dc.title Novel N4-like bacteriophages of pectobacterium atrosepticum en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Aidan Coffey, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 E-mail: aidan.coffey@cit.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder KU Leuven
dc.description.status Peer reviewed en
dc.identifier.journaltitle Pharmaceuticals en
dc.internal.IRISemailaddress aidan.coffey@cit.ie en
dc.identifier.articleid 45
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Infrastructure Programme/12/RI/2335 (2)/IE/ICP-HRMS mass spectrometer/


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© 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Except where otherwise noted, this item's license is described as © 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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