Bifidobacterium and Lactobacillus composition at species level and gut microbiota diversity in infants before 6 weeks
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Published version
Supplementary file 1
Date
2019-07-05
Authors
Yang, Bo
Chen, Yingqi
Stanton, Catherine
Ross, R. Paul
Lee, Yuan-Kun
Zhao, Jianxin
Zhang, Hao
Chen, Wei
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI AG
Published Version
Abstract
Our objective was to investigate the effects of different delivery and feeding modes on the gut microbiota composition of early infants with special emphasis on Bifidobacterium and Lactobacillus profiles at species level. 16S rRNA V3-V4 regions, bifidobacterial, and lactobacilli groEL genes from infant feces were sequenced by Illumina MiSeq. Gut microbiota abundance was significantly different, where standard vaginally delivered (SVD) and breast-fed (BF) groups were higher in comparison with caesarean section (CS), milk-powder-fed (MPF), and mixed-fed (MF) groups. The genus unclassified Enterobacteriaceae was dominant, followed by Bifidobacterium, which was highly abundant in SVD and BF groups. The dominant Bifidobacterium species in all groups were B. longum subsp. longum, B. longum subsp. infantis and B. animalis subsp. lactis. B. dentium and the diversity of Bifidobacterium in SVD and BF groups were significantly higher. For Lactobacillus profiles, L. rhamnosus and L. gasseri were dominant among all the groups, while Lactobacillus species in CS and MPF groups were more diverse. Functional predictions showed significant differences between delivery mode and feeding groups, such as phosphotransferase system as well as taurine and hypotaurine metabolism. In early infants with different delivery and feeding methods, gut microbiota—particularly bifidobacteria and lactobacilli communities—showed significant differences, with strong implications for physiological functions.
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Keywords
Gut microbiota , Bifidobacterium communities , Lactobacillus communities , Diversity , Infants , Functional prediction
Citation
Yang, B., Chen, Y., Stanton, C., Ross, R. P., Lee, Y.-K., Zhao, J., Zhang, H. and Chen, W. (2019) 'Bifidobacterium and Lactobacillus Composition at Species Level and Gut Microbiota Diversity in Infants before 6 Weeks', International Journal of Molecular Sciences, 20(13). (16pp.) 3306. DOI: 10.3390/ijms20133306