Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci

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Date
2020-12
Authors
Hill, Colin
O'Connor, Paula M.
Altermann, Eric
Day, Li
Hill, Colin
Stanton, Catherine
Ross, R. Paul
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Publisher
Springer Nature
Published Version
10.1038/s41598-020-70328-z
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Abstract
Streptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.
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Keywords
Streptococcus bovis , Streptococcus equinus , Gallocin D , Streptococcus gallolyticus LL009 , Vancomycin resistant enterococci
Citation
Hill, D., O’Connor, P. M., Altermann, E., Day, L., Hill, C., Stanton, C. and Ross, R. P. (2020) ‘Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci’, Scientific Reports, 10(1), 13431 (11pp). doi: 10.1038/s41598-020-70328-z
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https://hdl.handle.net/10468/14281
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Food and Nutritional Sciences - Journal Articles
APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles
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© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.