Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci

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dc.contributor.authorHill, Colin
dc.contributor.authorO'Connor, Paula M.
dc.contributor.authorAltermann, Eric
dc.contributor.authorDay, Li
dc.contributor.authorHill, Colin
dc.contributor.authorStanton, Catherine
dc.contributor.authorRoss, R. Paul
dc.contributor.funderTeagascen
dc.contributor.funderFood Processing for Health Longlife Projecten
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2023-03-03T16:19:24Z
dc.date.available2023-03-03T16:19:24Z
dc.date.issued2020-12
dc.date.updated2023-03-03T15:55:44Z
dc.description.abstractStreptococcus gallolyticus LL009 produces gallocin D, a narrow spectrum two component bacteriocin with potent activity against vancomycin-resistant enterococci. Gallocin D is distinct from gallocin A, a separate two component bacteriocin produced by S. gallolyticus. Although the gene clusters encoding gallocin A and gallocin D have a high degree of gene synteny, the structural genes are highly variable and appear to have undergone gene shuffling with other streptococcal species. Gallocin D was analysed in laboratory-based experiments. The mature peptides are 3,343 ± 1 Da and 3,019 ± 1 Da and could be readily synthesized and display activity against a vancomycin resistant Enterococcus strain EC300 with a MIC value of 1.56 µM. Importantly, these bacteriocins could contribute to the ability of S. gallolyticus to colonize the colon where they have been associated with colorectal cancer.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid13431en
dc.identifier.citationHill, D., O’Connor, P. M., Altermann, E., Day, L., Hill, C., Stanton, C. and Ross, R. P. (2020) ‘Extensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococci’, Scientific Reports, 10(1), 13431 (11pp). doi: 10.1038/s41598-020-70328-zen
dc.identifier.doi10.1038/s41598-020-70328-zen
dc.identifier.endpage11en
dc.identifier.issn2045-2322
dc.identifier.issued1en
dc.identifier.journaltitleScientific Reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/14281
dc.identifier.volume10en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.projectTeagasc (Walsh Fellowship Scheme)en
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectStreptococcus bovisen
dc.subjectStreptococcus equinusen
dc.subjectGallocin Den
dc.subjectStreptococcus gallolyticus LL009en
dc.subjectVancomycin resistant enterococcien
dc.titleExtensive bacteriocin gene shuffling in the Streptococcus bovis/Streptococcus equinus complex reveals gallocin D with activity against vancomycin resistant enterococcien
dc.typeArticle (peer-reviewed)en
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