Using hematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals.

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dc.contributor.authorMwakasungula, Solomon
dc.contributor.authorRougeron, Virginie
dc.contributor.authorArnathau, Céline
dc.contributor.authorBoundenga, Larson
dc.contributor.authorMiguel, Eve
dc.contributor.authorBoissière, Anne
dc.contributor.authorJiolle, Davy
dc.contributor.authorDurand, Patrick
dc.contributor.authorMsigwa, Alphonce
dc.contributor.authorMswata, Sarah
dc.contributor.authorOlotu, Ally
dc.contributor.authorSterkers, Yvon
dc.contributor.authorRoche, Benjamin
dc.contributor.authorKilleen, Gerard
dc.contributor.authorCerqueira, Frédérique
dc.contributor.authorBitome-Essono, Paul Yannick
dc.contributor.authorBretagnolle, François
dc.contributor.authorMasanja, Honorati
dc.contributor.authorPaupy, Christophe
dc.contributor.authorSumaye, Robert
dc.contributor.authorPrugnolle, Franck
dc.contributor.funderAgence Nationale de la Rechercheen
dc.contributor.funderHorizon 2020en
dc.date.accessioned2022-06-30T14:12:17Z
dc.date.available2022-06-30T14:12:17Z
dc.date.issued2022-06-22
dc.date.updated2022-06-29T15:18:10Z
dc.description.abstractMany emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities.en
dc.description.sponsorshipAgence Nationale de la Recherche (ANR-10-LABX-04-01; ANR-11-LABX-0024)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMwakasungula, S., Rougeron, V., Arnathau, C., Boundenga, L., Miguel, E., Boissière, A., Jiolle, D., Durand, P., Msigwa, A., Mswata, S., Olotu, A., Sterkers, Y., Roche, B., Killeen, G., Cerqueira, F., Bitome-Essono, P. Y., Bretagnolle, F., Masanja, H., Paupy, C., Sumaye, R. and Prugnolle, F. (2022) 'Using hematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals', Molecular Ecology Resources. doi: 10.1111/1755-0998.13670en
dc.identifier.doi10.1111/1755-0998.13670en
dc.identifier.eissn1755-0998
dc.identifier.issn755-098X
dc.identifier.journaltitleMolecular Ecology Resourcesen
dc.identifier.urihttps://hdl.handle.net/10468/13334
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::RIA/734548/EU/A global alliance for Zika virus control and prevention/ZIKAllianceen
dc.rights© 2022, John Wiley & Sons, Inc. This is the peer reviewed version of the following item: Mwakasungula, S., Rougeron, V., Arnathau, C., Boundenga, L., Miguel, E., Boissière, A., Jiolle, D., Durand, P., Msigwa, A., Mswata, S., Olotu, A., Sterkers, Y., Roche, B., Killeen, G., Cerqueira, F., Bitome-Essono, P. Y., Bretagnolle, F., Masanja, H., Paupy, C., Sumaye, R. and Prugnolle, F. (2022) 'Using hematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals', Molecular Ecology Resources, doi: 10.1111/1755-0998.13670, which has been published in final form at https://doi.org/10.1111/1755-0998.13670. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectInvertebrate-derived DNA (iDNA)en
dc.subjectWildlife infectious diseasesen
dc.subjectXenosurveillanceen
dc.subjectBlood mealsen
dc.subjectTsetse fliesen
dc.titleUsing hematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals.en
dc.typeArticle (peer-reviewed)en
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