Bacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin E

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dc.contributor.authorKamilari, Elenien
dc.contributor.authorO'Connor, Paula M.en
dc.contributor.authorde Farias, Felipe Micelien
dc.contributor.authorJohnson, C. N.en
dc.contributor.authorButtimer, Colinen
dc.contributor.authorDeliephan, Aiswariyaen
dc.contributor.authorHill, Daraghen
dc.contributor.authorFursenko, Oxanaen
dc.contributor.authorWiese, Jonathanen
dc.contributor.authorStanton, Catherineen
dc.contributor.authorHill, Colinen
dc.contributor.authorRoss, R. Paulen
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2025-08-28T13:11:20Z
dc.date.available2025-08-28T13:11:20Z
dc.date.issued2025-01-31en
dc.description.abstractBacillus safensis APC 4099, isolated from bees’ gut, has been identified as a promising candidate for food biopreservation. Antimicrobial activity screening revealed a broad-spectrum inhibition potential, ranging from gram-positive pathogenic bacteria to fungi responsible for food spoilage. Genomic analysis identified biosynthetic gene clusters coding for several antimicrobial peptides and secondary metabolites. Specifically, a novel, anionic, 6 kDa circular bacteriocin, named safencin E, was detected, showing 52.5% similarity to butyrivibriocin AR10. Additionally, gene clusters coding for the biosynthesis of bacteriocins such as pumilarin and plantazolicin and biosynthetic pathways for secondary metabolites, including pumilacidin A, bacilysin, and bacillibactin, were identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis detected molecular masses correlating to safencin E, plantazolicin, pumilarin, and pumilacidin A from the cell-free supernatant, cell extracts, or both. Overall, the broad-spectrum antimicrobial activity of B. safensis APC 4099 indicates that this strain is a promising candidate for the biological control of food ecosystems and thus has the potential to enhance food safety.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide01942-24en
dc.identifier.citationKamilari, E., O'Connor, P.M., Farias, F.M.D., Johnson, C.N., Buttimer, C., Deliephan, A., Hill, D., Fursenko, O., Wiese, J., Stanton, C., Hill, C. and Ross, R.P. (2025) 'Bacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin E', Applied and Environmental Microbiology, 91(1), e01942-24 (20pp). https://doi.org/10.1128/aem.01942-24en
dc.identifier.doi10.1128/aem.01942-24en
dc.identifier.eissn1098-5336en
dc.identifier.endpage20en
dc.identifier.issn0099-2240en
dc.identifier.issued1en
dc.identifier.journaltitleApplied and Environmental Microbiologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/17809
dc.identifier.volume91en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/Research Centres Programme/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2024, Kamilari et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectBacillus safensisen
dc.subjectAntimicrobial peptidesen
dc.subjectCircular bacteriocinen
dc.subjectSafencin Een
dc.subjectPumilarinen
dc.subjectPlantazolicinen
dc.subjectPumilacidinen
dc.subjectBacilysinen
dc.subjectBacillibactinen
dc.subjectFood biopreservationen
dc.subject~Microbiology - Journal Articles~en
dc.titleBacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin Een
dc.typeArticle (peer-reviewed)en
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