Bacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin E
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Date
2025-01-31
Authors
Kamilari, Eleni
O’Connor, Paula M.
de Farias, Felipe Miceli
Johnson, C. N.
Buttimer, Colin
Deliephan, Aiswariya
Hill, Daragh
Fursenko, Oxana
Wiese, Jonathan
Stanton, Catherine
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Published Version
Abstract
Bacillus safensis APC 4099, isolated from bees’ gut, has been identified as a promising candidate for food biopreservation. Antimicrobial activity screening revealed a broad-spectrum inhibition potential, ranging from gram-positive pathogenic bacteria to fungi responsible for food spoilage. Genomic analysis identified biosynthetic gene clusters coding for several antimicrobial peptides and secondary metabolites. Specifically, a novel, anionic, 6 kDa circular bacteriocin, named safencin E, was detected, showing 52.5% similarity to butyrivibriocin AR10. Additionally, gene clusters coding for the biosynthesis of bacteriocins such as pumilarin and plantazolicin and biosynthetic pathways for secondary metabolites, including pumilacidin A, bacilysin, and bacillibactin, were identified. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis detected molecular masses correlating to safencin E, plantazolicin, pumilarin, and pumilacidin A from the cell-free supernatant, cell extracts, or both. Overall, the broad-spectrum antimicrobial activity of B. safensis APC 4099 indicates that this strain is a promising candidate for the biological control of food ecosystems and thus has the potential to enhance food safety.
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Keywords
Bacillus safensis , Antimicrobial peptides , Circular bacteriocin , Safencin E , Pumilarin , Plantazolicin , Pumilacidin , Bacilysin , Bacillibactin , Food biopreservation
Citation
Kamilari, E., O’Connor, P.M., Farias, F.M.D., Johnson, C.N., Buttimer, C., Deliephan, A., Hill, D., Fursenko, O., Wiese, J., Stanton, C., Hill, C. and Ross, R.P. (2025) 'Bacillus safensis APC 4099 has broad-spectrum antimicrobial activity against both bacteria and fungi and produces several antimicrobial peptides, including the novel circular bacteriocin safencin E', Applied and Environmental Microbiology, 91(1), e01942-24 (20pp). https://doi.org/10.1128/aem.01942-24