Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages

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Sprotte, Sabrina
Fagbemigun, Olakunle
Brinks, Erik
Cho, Gyu-Sung
Casey, Eoghan
Oguntoyinbo, Folarin A.
Neve, Horst
Mahony, Jennifer
van Sinderen, Douwe
Franz, Charles M.A.P.
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Elsevier B.V.
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A novel Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from the Nigerian fermented milk product nono. The phage possesses a long and thin, non-contractile tail and an isometric head, indicating that it belongs to the Siphoviridae family. A neck passage structure (`collar`), previously hypothesized to be encoded by two genes located in the Lactobacillus delbrueckii phage LL-K insertion sequence (KIS) element, as well as in two additional Lb. delbrueckii phages Ld17 and Ld25A, could also be observed on an estimated 1–5% of phage particles by transmission electron microscopy. However, neither mapping of high throughput sequencing data to KIS element genes from Lb. delbrueckii phages LL-K, Ld17 and Ld25A nor PCR amplification of the KIS element genes could corroborate the presence of these genes in the PMBT4 genome. The PMBT4 genome consists of 31,399 bp with a mol% GC content of 41.6 and exhibits high (95–96%) sequence homologies to Lb. delbrueckii phages c5, Ld3, Ld25A and Ld17, which assigned PMBT4 as a new member of this genus, i.e. the Cequinquevirus genus.
Lactobacillus delbrueckii phage , Genome , Group b , KIS element
Sprotte, S., Fagbemigun, O.,Brinks, E., Cho, G.-S., Casey, E., Oguntoyinbo, F. A., Neve, H., Mahony, J., van Sinderen, D. and Franz, C. M.A.P. (2021) 'Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages', Virus Research, 308, 198635 (7pp). doi: 10.1016/j.virusres.2021.198635
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