Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages

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dc.contributor.authorSprotte, Sabrina
dc.contributor.authorFagbemigun, Olakunle
dc.contributor.authorBrinks, Erik
dc.contributor.authorCho, Gyu-Sung
dc.contributor.authorCasey, Eoghan
dc.contributor.authorOguntoyinbo, Folarin A.
dc.contributor.authorNeve, Horst
dc.contributor.authorMahony, Jennifer
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorFranz, Charles M.A.P.
dc.contributor.funderAlexander von Humboldt-Stiftungen
dc.date.accessioned2022-01-17T15:43:09Z
dc.date.available2022-01-17T15:43:09Z
dc.date.issued2021-11-19
dc.date.updated2022-01-17T15:27:02Z
dc.description.abstractA novel Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from the Nigerian fermented milk product nono. The phage possesses a long and thin, non-contractile tail and an isometric head, indicating that it belongs to the Siphoviridae family. A neck passage structure (`collar`), previously hypothesized to be encoded by two genes located in the Lactobacillus delbrueckii phage LL-K insertion sequence (KIS) element, as well as in two additional Lb. delbrueckii phages Ld17 and Ld25A, could also be observed on an estimated 1–5% of phage particles by transmission electron microscopy. However, neither mapping of high throughput sequencing data to KIS element genes from Lb. delbrueckii phages LL-K, Ld17 and Ld25A nor PCR amplification of the KIS element genes could corroborate the presence of these genes in the PMBT4 genome. The PMBT4 genome consists of 31,399 bp with a mol% GC content of 41.6 and exhibits high (95–96%) sequence homologies to Lb. delbrueckii phages c5, Ld3, Ld25A and Ld17, which assigned PMBT4 as a new member of this genus, i.e. the Cequinquevirus genus.en
dc.description.sponsorshipAlexander von Humboldt Stiftung (grant number 3.4-IPDEU/1,154,993)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid198635en
dc.identifier.citationSprotte, S., Fagbemigun, O.,Brinks, E., Cho, G.-S., Casey, E., Oguntoyinbo, F. A., Neve, H., Mahony, J., van Sinderen, D. and Franz, C. M.A.P. (2021) 'Novel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phages', Virus Research, 308, 198635 (7pp). doi: 10.1016/j.virusres.2021.198635en
dc.identifier.doi10.1016/j.virusres.2021.198635en
dc.identifier.endpage7en
dc.identifier.issn1872-7492
dc.identifier.issn0168-1702
dc.identifier.journaltitleVirus Researchen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/12399
dc.identifier.volume308en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.rights© 2021, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectLactobacillus delbrueckii phageen
dc.subjectGenomeen
dc.subjectGroup ben
dc.subjectKIS elementen
dc.titleNovel Siphoviridae phage PMBT4 belonging to the group b Lactobacillus delbrueckii subsp. bulgaricus phagesen
dc.typeArticle (peer-reviewed)en
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