Enhanced dark hydrogen fermentation of Enterobacter aerogenes/HoxEFUYH with carbon cloth
| dc.contributor.author | Cheng, Jun | |
| dc.contributor.author | Li, Hui | |
| dc.contributor.author | Zhang, Jiabei | |
| dc.contributor.author | Ding, Lingkan | |
| dc.contributor.author | Ye, Qing | |
| dc.contributor.author | Lin, Richen | |
| dc.contributor.funder | State Key Laboratory of Protein and Plant Gene Research | en |
| dc.contributor.funder | Science and Technology Department of Zhejiang Province | en |
| dc.contributor.funder | H2020 Marie Skłodowska-Curie Actions | en |
| dc.date.accessioned | 2019-01-18T12:20:39Z | |
| dc.date.available | 2019-01-18T12:20:39Z | |
| dc.date.issued | 2019-01-04 | |
| dc.description.abstract | Long-range extracellular electron transfer through microbial nanowires is critical for efficient bacterial behaviors. The application of carbon cloth on the dark hydrogen fermentation using transgenic Enterobacter aerogenes (E. aerogenes/HoxEFUYH) was first proposed to enhance hydrogen production from glucose. Scanning electron microscopy images showed that the microbial nanowires between E. aerogenes/HoxEFUYH cells almost vanished due to the presence of carbon cloth. Approximately 59.1% of microorganisms concentrated in biofilms on the surface of carbon cloth, which probably promoted the intercellular electron transfer. The results from Fourier transform infrared spectra and Excitation Emission Matrix spectra indicated that carbon cloth biofilms primarily included polysaccharide and protein. Moreover, the fluorophore of biofilms (88.1%) was much higher than that of supernatant (11.9%). The analysis of soluble metabolic degradation byproducts revealed that carbon cloth selectively enhanced the acetate pathway (C6H12O6+2H2O→2CH3COOH+2CO2+4H2), but weakened the ethanol pathway (C6H12O6→2C2H5OH+2CO2). With 1.0 g/L carbon cloth, the hydrogen yield increased by 26.6% to 242 mL/g, and the corresponding peak hydrogen production rate increased by 60.3%. | en |
| dc.description.sponsorship | State Key Laboratory of Protein and Plant Gene Research (National Key Research andDevelopment Program-China (2016YFE0117900)); Science and Technology Department of Zhejiang Province (Zhejiang Provincial Key Research and Development Program-China (2017C0400)); | en |
| dc.description.status | Peer reviewed | en |
| dc.description.version | Accepted Version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Cheng, J., Li, H., Zhang, J., Ding, L., Ye, Q. and Lin, R. (2019) 'Enhanced dark hydrogen fermentation of Enterobacter aerogenes/HoxEFUYH with carbon cloth', International Journal of Hydrogen Energy, In Press, doi: 10.1016/j.ijhydene.2018.12.080 | en |
| dc.identifier.doi | 10.1016/j.ijhydene.2018.12.080 | |
| dc.identifier.endpage | 32 | en |
| dc.identifier.issn | 0360-3199 | |
| dc.identifier.journaltitle | International Journal of Hydrogen Energy | en |
| dc.identifier.startpage | 1 | en |
| dc.identifier.uri | https://hdl.handle.net/10468/7317 | |
| dc.language.iso | en | en |
| dc.publisher | Elsevier | en |
| dc.relation.project | info:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-ST/797259/EU/Direct Interspecies Electron Transfer in advanced anaerobic digestion system for gaseous transport biofuel production/DIET | en |
| dc.relation.uri | http://www.sciencedirect.com/science/article/pii/S0360319918340448 | |
| dc.rights | © 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
| dc.subject | Electro-conductive carbon cloth | en |
| dc.subject | Hydrogen fermentation | en |
| dc.subject | Transgenic Enterobacter aerogenes | en |
| dc.title | Enhanced dark hydrogen fermentation of Enterobacter aerogenes/HoxEFUYH with carbon cloth | en |
| dc.type | Article (peer-reviewed) | en |
