Lost in translation? The potential psychobiotic Lactobacillus rhamnosus (JB-1) fails to modulate stress or cognitive performance in healthy male subjects

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dc.contributor.authorKelly, John R.
dc.contributor.authorAllen, Andrew P.
dc.contributor.authorTemko, Andriy
dc.contributor.authorHutch, William
dc.contributor.authorKennedy, Paul J.
dc.contributor.authorFarid, Niloufar
dc.contributor.authorMurphy, Eileen F.
dc.contributor.authorBoylan, Geraldine B.
dc.contributor.authorBienenstock, John
dc.contributor.authorCryan, John F.
dc.contributor.authorClarke, Gerard
dc.contributor.authorDinan, Timothy G.
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHealth Research Boarden
dc.contributor.funderEuropean Commissionen
dc.contributor.funderSeventh Framework Programmeen
dc.contributor.funderBrain and Behaviour Research Foundation, United Statesen
dc.date.accessioned2016-11-28T16:00:26Z
dc.date.available2016-11-28T16:00:26Z
dc.date.issued2016-11-16
dc.date.updated2016-11-28T15:43:46Z
dc.description.abstractBackground: Preclinical studies have identified certain probiotics as psychobiotics a live microorganisms with a potential mental health benefit. Lactobacillus rhamnosus (JB-1) has been shown to reduce stress-related behaviour, corticosterone release and alter central expression of GABA receptors in an anxious mouse strain. However, it is unclear if this single putative psychobiotic strain has psychotropic activity in humans. Consequently, we aimed to examine if these promising preclinical findings could be translated to healthy human volunteers. Objectives: To determine the impact of L. rhamnosus on stress-related behaviours, physiology, inflammatory response, cognitive performance and brain activity patterns in healthy male participants. An 8 week, randomized, placebo-controlled, cross-over design was employed. Twenty-nine healthy male volunteers participated. Participants completed self-report stress measures, cognitive assessments and resting electroencephalography (EEG). Plasma IL10, IL1β, IL6, IL8 and TNFα levels and whole blood Toll-like 4 (TLR-4) agonist-induced cytokine release were determined by multiplex ELISA. Salivary cortisol was determined by ELISA and subjective stress measures were assessed before, during and after a socially evaluated cold pressor test (SECPT). Results: There was no overall effect of probiotic treatment on measures of mood, anxiety, stress or sleep quality and no significant effect of probiotic over placebo on subjective stress measures, or the HPA response to the SECPT. Visuospatial memory performance, attention switching, rapid visual information processing, emotion recognition and associated EEG measures did not show improvement over placebo. No significant anti-inflammatory effects were seen as assessed by basal and stimulated cytokine levels. Conclusions: L. rhamnosus was not superior to placebo in modifying stress-related measures, HPA response, inflammation or cognitive performance in healthy male participants. These findings highlight the challenges associated with moving promising preclinical studies, conducted in an anxious mouse strain, to healthy human participants. Future interventional studies investigating the effect of this psychobiotic in populations with stress-related disorders are required.en
dc.description.sponsorshipScience Foundation Ireland (SFI Grant Number SFI/12/RC/2273); Health Research Board (HRB Health Research Awards (grant nos. HRA_POR/2011/23; HRA_POR/2012/32; HRA-POR-2-14-647); European Commission (EU GRANT 613979 (MYNEWGUT FP7-KBBE-2013-7); Brain and Behaviour Research Foundation, United States (NARSAD Young Investigator Grant Number 20771).en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationKelly, J. R., Allen, A. P., Temko, A., Hutch, W., Kennedy, P. J., Farid, N., Murphy, E., Boylan, G., Bienenstock, J., Cryan, J. F., Clarke, G. and Dinan, T. G. (2017) 'Lost in translation? The potential psychobiotic Lactobacillus rhamnosus (JB-1) fails to modulate stress or cognitive performance in healthy male subjects', Brain, Behavior, and Immunity, 61, pp. 50-59. doi: 10.1016/j.bbi.2016.11.018en
dc.identifier.doi10.1016/j.bbi.2016.11.018
dc.identifier.endpage59
dc.identifier.issn0889-1591
dc.identifier.journaltitleBrain, Behavior, And Immunityen
dc.identifier.startpage50
dc.identifier.urihttps://hdl.handle.net/10468/3316
dc.identifier.volume61
dc.language.isoenen
dc.publisherElsevieren
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/613979/EU/Microbiome Influence on Energy balance and Brain Development-Function Put into Action to Tackle Diet-related Diseases and Behavior./MYNEWGUTen
dc.rights© 2016, Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectPsychobioticen
dc.subjectBrain-gut axisen
dc.subjectStressen
dc.subjectCognitionen
dc.subjectMemoryen
dc.subjectEEGen
dc.titleLost in translation? The potential psychobiotic Lactobacillus rhamnosus (JB-1) fails to modulate stress or cognitive performance in healthy male subjectsen
dc.typeArticle (peer-reviewed)en
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