dc.contributor.author	Ortiz Charneco, Guillermo	en
dc.contributor.author	Kelleher, Philip	en
dc.contributor.author	Buivydas, Andrius	en
dc.contributor.author	Dashko, Sofia,	en
dc.contributor.author	de Waal, Paul P.	en
dc.contributor.author	van Peij, Noël N. M. E.	en
dc.contributor.author	Roberts, Richard John	en
dc.contributor.author	Mahony, Jennifer	en
dc.contributor.author	van Sinderen, Douwe	en
dc.contributor.funder	Science Foundation Ireland
dc.date.accessioned	2023-08-16T09:59:34Z
dc.date.available	2023-08-16T10:05:09Z	en
dc.date.available	2023-08-16T09:59:34Z
dc.date.issued	2023-06	en
dc.date.updated	2023-08-16T09:05:13Z	en
dc.description.abstract	Plasmid pUC11B is a 49.3-kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01-like conjugation system and tetracycline-resistance. In this study, we demonstrate that this plasmid can be conjugated at high frequencies to recipient strains. Mutational analysis of the 22 genes encompassing the presumed pUC11B conjugation cluster revealed the presence of several genes with essential conjugation functions, as well as a gene, trsR, encoding a putative transcriptional repressor of this conjugation cluster. Furthermore, plasmid pUC11B encodes an anti-restriction protein, TrsAR, which facilitates higher conjugation frequencies when pUC11B is transferred into recipient strains containing Type II or Type III RM systems. These findings demonstrate how RM mechanisms can be circumvented when they act as a biological barrier for conjugation events.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Ortiz Charneco, G., Kelleher, P., Buivydas, A., Dashko, S., De Waal, P. P., Van Peij, N. N. M. E., Roberts, R. J., Mahony, J. and Van Sinderen, D. (2023) ‘Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system’, Microbial Biotechnology, 16(6), pp. 1250–1263. doi: 10.1111/1751-7915.14221
dc.identifier.doi	10.1111/1751-7915.14221	en
dc.identifier.endpage	1263
dc.identifier.issued	6
dc.identifier.journaltitle	Microbial Biotechnology
dc.identifier.startpage	1250
dc.identifier.uri	https://hdl.handle.net/10468/14836
dc.identifier.volume	16
dc.language.iso	en	en
dc.publisher	John Wiley & Sons, Inc.
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Spokes Programme::Rolling Call/17/SP/4678/IE/BacTrans – Natural DNA Transfer Systems for Bacterial Starter Cultures/
dc.rights	© 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Plasmid pUC11B
dc.subject	Mutational analysisp
dc.subject	UC11B conjugation cluster
dc.subject	RM mechanisms
dc.title	Delineation of a lactococcal conjugation system reveals a restriction-modification evasion system
dc.type	Article (peer-reviewed)

Delineation of a lactococcal conjugation system reveals a restriction-modification evasion system

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