Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003

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dc.contributor.authorChristiaen, Steven E. A.
dc.contributor.authorO'Connell Motherway, Mary
dc.contributor.authorBottacini, Francesca
dc.contributor.authorLanigan, Noreen
dc.contributor.authorCasey, Pat G.
dc.contributor.authorHuys, Geert
dc.contributor.authorNelis, Hans J.
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorCoenye, Tom
dc.contributor.funderBelgian Science Policy Office
dc.contributor.funderAgentschap voor Innovatie door Wetenschap en Technologie, Belgium
dc.contributor.funderHealth Research Board
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderIrish Research Council for Science Engineering and Technology
dc.date.accessioned2016-02-17T11:44:40Z
dc.date.available2016-02-17T11:44:40Z
dc.date.issued2014
dc.description.abstractIn the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.en
dc.description.sponsorshipBelgian Science Policy Office (Interuniversity Attraction Poles Programme); Health Research Board (Postdoctoral Fellowship PDTM/20011/9); Irish Research Council for Science Engineering and Technology (Postgraduate Fellowship); Science Foundation Ireland (SFI Grant No. 07/CE/B1368; SFI/12/RC/2273)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide98111
dc.identifier.citationChristiaen SEA, O'Connell Motherway M, Bottacini F, Lanigan N, Casey PG, Huys G, et al. (2014) Autoinducer-2 Plays a Crucial Role in Gut Colonization and Probiotic Functionality of Bifidobacterium breve UCC2003. PLoS ONE 9(5): e98111. doi:10.1371/journal.pone.0098111
dc.identifier.doi10.1371/journal.pone.0098111
dc.identifier.issn1932-6203
dc.identifier.issued5en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2338
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Christiaen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectInflammatory bowel diseaseen
dc.subjectLactobacillus rhamnosus GGen
dc.subjectDna-microarray dataen
dc.subjectQuorum sensing roleen
dc.subjectCaenorhabditis elegansen
dc.subjectNecrotizing enterocolitisen
dc.subjectGene expressionen
dc.subjectPseudomonas aeruginosaen
dc.subjectSalmonella typhimuriumen
dc.subjectEscherichia colien
dc.titleAutoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003en
dc.typeArticle (peer-reviewed)en
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