Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential

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dc.contributor.authorLynch, Kieran M.
dc.contributor.authorStrain, Conall R.
dc.contributor.authorJohnson, Crystal
dc.contributor.authorPatangia, Dhrati
dc.contributor.authorStanton, Catherine
dc.contributor.authorKoc, Fatma
dc.contributor.authorGil-Martinez, Jorge
dc.contributor.authorO'Riordan, Patrick
dc.contributor.authorSahin, Aylin W.
dc.contributor.authorRoss, R. Paul
dc.contributor.authorArendt, Elke K.
dc.contributor.funderIrish Research eLibraryen
dc.date.accessioned2022-03-07T16:16:10Z
dc.date.available2022-03-07T16:16:10Z
dc.date.issued2021-12
dc.date.updated2022-03-07T15:33:08Z
dc.description.abstractPurpose: Brewers' spent grain (BSG) represents the largest by-product of the brewing industry. Its utilisation as an animal feed has become less practical today; however, its high fibre and protein content make it a promising untapped resource for human nutrition. BSG contains mainly insoluble fibre. This fibre, along with protein, is trapped with the complex lignocellulosic cell structure and must be solubilised to release components which may be beneficial to health through modulation of the gut microbiota. Methods: In this study, the application of a simultaneous saccharification and fermentation process for the extraction and solubilisation of arabinoxylan from BSG is demonstrated. Results: Processing of the BSG was varied to modulate the physicochemical and molecular characteristic of the released arabinoxylan. The maximum level of arabinoxylan solubilisation achieved was approximately 21%, compared to the unprocessed BSG which contained no soluble arabinoxylan (AX). Concentration of the solubilised material produced a sample containing 99% soluble AX. Samples were investigated for their microbiome modulating capacity in in-vitro faecal fermentation trials. Many samples promoted increased Lactobacillus levels (approx. twofold). One sample that contained the highest level of soluble AX was shown to be bifidogenic, increasing the levels of this genus approx. 3.5-fold as well as acetate (p = 0.018) and propionate (p< 0.001) production. Conclusion: The findings indicate that AX extracted from BSG has prebiotic potential. The demonstration that BSG is a source of functional fibre is a promising step towards the application of this brewing side-stream as a functional food ingredient for human nutrition.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLynch, K. M., Strain, C. R., Johnson, C., Patangia, D., Stanton, C., Koc, F., Gil-Martinez, J., O'Riordan, P., Sahin, A. W., Ross, R. P. and Arendt, E. K. (2021) 'Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential', European Journal of Nutrition, 60 (8), pp. 4393-4411. doi: 10.1007/s00394-021-02570-8en
dc.identifier.doi10.1007/s00394-021-02570-8en
dc.identifier.endpage4411en
dc.identifier.issn1436-6207
dc.identifier.issued8en
dc.identifier.journaltitleEuropean Journal of Nutritionen
dc.identifier.startpage4393en
dc.identifier.urihttps://hdl.handle.net/10468/12805
dc.identifier.volume60en
dc.language.isoenen
dc.publisherSpringer International Publishing AGen
dc.rights© 2021, The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectIn-vitro fermentationen
dc.subjectWheat bran extracten
dc.subjectChain fatty-acidsen
dc.subjectGut microbiotaen
dc.subjectSoluble arabinoxylansen
dc.subjectEnzymatic-hydrolysisen
dc.subjectBarrier functionen
dc.subjectDietary-fibersen
dc.subjectOligosaccharidesen
dc.subjectHealthen
dc.titleExtraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potentialen
dc.typeArticle (peer-reviewed)en
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