Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens

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Table S1 Summary table of raw Illumina sequences for each bumblebee queen.
imb12618-sup-0004-tables2.xlsx(48.63 KB)
Table S2 Differentially expressed genes (DEGs) within Bombus terrestris queens in response to Sphaerularia bombi infection for each time-point.
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Table S3 Enriched gene ontology terms within host genes affected by Sphaerularia bombi infection.
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Figure S1 Principal component analysis for normalized gene-level estimated counts for transcriptomic profiles of diapause (blue) and postdiapause queens (grey).
Date
2019-09-30
Authors
Colgan, T. J.
Carolan, J. C.
Sumner, S.
Blaxter, M. L.
Brown, M. J. F.
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John Wiley & Sons, Inc.
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Abstract
Parasitism can result in dramatic changes in host phenotype, which are themselves underpinned by genes and their expression. Understanding how hosts respond at the molecular level to parasites can therefore reveal the molecular architecture of an altered host phenotype. The entomoparasitic nematode Sphaerularia bombi is a parasite of bumblebee (Bombus) hosts where it induces complex behavioural changes and host castration. To examine this interaction at the molecular level, we performed genome-wide transcriptional profiling using RNA-Sequencing (RNA-Seq) of S. bombi-infected Bombus terrestris queens at two critical time-points: during and just after overwintering diapause. We found that infection by S. bombi affects the transcription of genes underlying host biological processes associated with energy usage, translation, and circadian rhythm. We also found that the parasite affects the expression of immune genes, including members of the Toll signalling pathway providing evidence for a novel interaction between the parasite and the host immune response. Taken together, our results identify host biological processes and genes affected by an entomoparasitic nematode providing the first steps towards a molecular understanding of this ecologically important host–parasite interaction.
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Pollinator health , Insect immunity , Molecular parasitism , Extended phenotype
Citation
Colgan, T. J., Carolan, J. C., Sumner, S., Blaxter, M. L. and Brown, M. J. F. (2020) 'Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens', Insect Molecular Biology, 29(2), pp. 170-182. doi: 10.1111/imb.12618
Copyright
© 2019, The Royal Entomological Society. Published by John Wiley & Sons, Inc. This is the peer reviewed version of the following item: Colgan, T. J., Carolan, J. C., Sumner, S., Blaxter, M. L. and Brown, M. J. F. (2020) 'Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens', Insect Molecular Biology, 29(2), pp. 170-182, doi: 10.1111/imb.12618, which has been published in final form at https://doi.org/10.1111/imb.12618. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.