dc.contributor.author	Colgan, T. J.	en
dc.contributor.author	Carolan, J. C.	en
dc.contributor.author	Sumner, S.	en
dc.contributor.author	Blaxter, M. L.	en
dc.contributor.author	Brown, M. J. F.	en
dc.contributor.funder	Science Foundation Ireland	en
dc.date.accessioned	2023-10-19T13:30:50Z
dc.date.available	2023-10-19T13:30:50Z
dc.date.issued	2019-09-30	en
dc.description.abstract	Parasitism can result in dramatic changes in host phenotype, which are themselves underpinned by genes and their expression. Understanding how hosts respond at the molecular level to parasites can therefore reveal the molecular architecture of an altered host phenotype. The entomoparasitic nematode Sphaerularia bombi is a parasite of bumblebee (Bombus) hosts where it induces complex behavioural changes and host castration. To examine this interaction at the molecular level, we performed genome-wide transcriptional profiling using RNA-Sequencing (RNA-Seq) of S. bombi-infected Bombus terrestris queens at two critical time-points: during and just after overwintering diapause. We found that infection by S. bombi affects the transcription of genes underlying host biological processes associated with energy usage, translation, and circadian rhythm. We also found that the parasite affects the expression of immune genes, including members of the Toll signalling pathway providing evidence for a novel interaction between the parasite and the host immune response. Taken together, our results identify host biological processes and genes affected by an entomoparasitic nematode providing the first steps towards a molecular understanding of this ecologically important host–parasite interaction.	en
dc.description.sponsorship	Science Foundation Ireland (Grant EEEOBF131)	en
dc.description.status	Peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Colgan, T. J., Carolan, J. C., Sumner, S., Blaxter, M. L. and Brown, M. J. F. (2020) 'Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens', Insect Molecular Biology, 29(2), pp. 170-182. doi: 10.1111/imb.12618	en
dc.identifier.doi	10.1111/imb.12618	en
dc.identifier.eissn	1365-2583	en
dc.identifier.endpage	182	en
dc.identifier.issn	0962-1075	en
dc.identifier.issued	2	en
dc.identifier.journaltitle	Insect Molecular Biology	en
dc.identifier.startpage	170	en
dc.identifier.uri	https://hdl.handle.net/10468/15137
dc.identifier.volume	29	en
dc.language.iso	en	en
dc.publisher	John Wiley & Sons, Inc.	en
dc.rights	© 2019, The Royal Entomological Society. Published by John Wiley & Sons, Inc. This is the peer reviewed version of the following item: Colgan, T. J., Carolan, J. C., Sumner, S., Blaxter, M. L. and Brown, M. J. F. (2020) 'Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens', Insect Molecular Biology, 29(2), pp. 170-182, doi: 10.1111/imb.12618, which has been published in final form at https://doi.org/10.1111/imb.12618. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.	en
dc.subject	Pollinator health	en
dc.subject	Insect immunity	en
dc.subject	Molecular parasitism	en
dc.subject	Extended phenotype	en
dc.title	Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens	en
dc.type	Article (peer-reviewed)	en
oaire.citation.issue	2	en
oaire.citation.volume	29	en

Infection by the castrating parasitic nematode Sphaerularia bombi changes gene expression in Bombus terrestris bumblebee queens

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