Sex-dependent associations between addiction-related behaviors and the microbiome in outbred rats

dc.contributor.authorPeterson, Veronica L.
dc.contributor.authorRichards, Jerry B.
dc.contributor.authorMeyer, Paul J.
dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.authorTripi, Jordan A.
dc.contributor.authorKing, Christopher P.
dc.contributor.authorPolesskaya, Oksana
dc.contributor.authorBaud, Amelie
dc.contributor.authorChitre, Apurva S.
dc.contributor.authorBastiaanssen, Thomaz F. S.
dc.contributor.authorWoods, Leah Solberg
dc.contributor.authorCrispie, Fiona
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorPalmer, Abraham A.
dc.contributor.authorCryan, John F.
dc.contributor.funderNational Institutes for Drug Abuseen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderWellcome Trusten
dc.date.accessioned2022-03-25T16:19:03Z
dc.date.available2022-03-25T16:19:03Z
dc.date.issued2020-05
dc.date.updated2022-03-25T15:43:19Z
dc.description.abstractBackground: Multiple factors contribute to the etiology of addiction, including genetics, sex, and a number of addiction-related behavioral traits. One behavioral trait where individuals assign incentive salience to food stimuli (â sign-trackersâ , ST) are more impulsive compared to those that do not (â goal-trackersâ , GT), as well as more sensitive to drugs and drug stimuli. Furthermore, this GT/ST phenotype predicts differences in other behavioral measures. Recent studies have implicated the gut microbiota as a key regulator of brain and behavior, and have shown that many microbiota-associated changes occur in a sex-dependent manner. However, few studies have examined how the microbiome might influence addiction-related behaviors. To this end, we sought to determine if gut microbiome composition was correlated with addiction-related behaviors determined by the GT/ST phenotype. Methods: Outbred male (N=101) and female (N=101) heterogeneous stock rats underwent a series of behavioral tests measuring impulsivity, attention, reward-learning, incentive salience, and locomotor response. Cecal microbiome composition was estimated using 16S rRNA gene amplicon sequencing. Behavior and microbiome were characterized and correlated with behavioral phenotypes. Robust sex differences were observed in both behavior and microbiome; further analyses were conducted within sex using the pre-established goal/sign-tracking (GT/ST) phenotype and partial least squares differential analysis (PLS-DA) clustered behavioral phenotype. Results: Overall microbiome composition was not associated to the GT/ST phenotype. However, microbial alpha diversity was significantly decreased in female STs. On the other hand, a measure of impulsivity had many significant correlations to microbiome in both males and females. Several measures of impulsivity were correlated with the genus Barnesiella in females. Female STs had notable correlations between microbiome and attentional deficient. In both males and females, many measures were correlated with the bacterial families Ruminocococcaceae and Lachnospiraceae. Conclusions: These data demonstrate correlations between several addiction-related behaviors and the microbiome specific to sex.en
dc.description.sponsorshipNational Institutes for Drug Abuse (Grant Number P50DA037844); Wellcome Trust (Grant Number 105941/Z/14/Z)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationPeterson, V. L., Richards, J. B., Meyer, P. J., Cabrera-Rubio, R., Tripi, J. A., King, C. P., Polesskaya, O., Baud, A., Chitre, A. S., Bastiaanssen, T. F. S., Woods, L. S., Crispie, F., Dinan, T. G., Cotter, P. D., Palmer, A. A. and Cryan, John F. (2020) 'Sex-dependent associations between addiction-related behaviors and the microbiome in outbred rats', Ebiomedicine, 55, 102769 (13pp). doi: 10.1016/j.ebiom.2020.102769en
dc.identifier.doi10.1016/j.ebiom.2020.102769en
dc.identifier.endpage13en
dc.identifier.issn2352-3964
dc.identifier.journaltitleEbiomedicineen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/12996
dc.identifier.volume55en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/en
dc.rights© 2020 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectAddictionen
dc.subjectGut-brain axisen
dc.subjectMicrobiomeen
dc.subjectSexen
dc.subjectSign-trackeren
dc.titleSex-dependent associations between addiction-related behaviors and the microbiome in outbred ratsen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
1-s2.0-S2352396420301444-main_(1).pdf
Size:
3.19 MB
Format:
Adobe Portable Document Format
Description:
Published Version
Loading...
Thumbnail Image
Name:
1-s2.0-S2352396420301444-mmc1.docx
Size:
11.1 MB
Format:
Microsoft Word XML
Description:
Supplementary Documentation
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description: