Targeting the EP1 receptor reduces Fas ligand expression and increases the antitumor immune response in an in vivo model of colon cancer

dc.contributor.authorO'Callaghan, Grace
dc.contributor.authorRyan, Aideen E.
dc.contributor.authorNeary, Peter M.
dc.contributor.authorO'Mahony, Caitlin
dc.contributor.authorShanahan, Fergus
dc.contributor.authorHouston, Aileen M.
dc.contributor.funderHealth Research Boarden
dc.contributor.funderIrish Cancer Societyen
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2013-07-31T14:02:30Z
dc.date.available2013-07-31T14:02:30Z
dc.date.issued2013-08
dc.date.updated2013-07-03T08:00:58Z
dc.description.abstractDespite studies demonstrating that inhibition of cyclooxygenase-2 (COX-2)-derived prostaglandin E2 (PGE2) has significant chemotherapeutic benefits in vitro and in vivo, inhibition of COX enzymes is associated with serious gastrointestinal and cardiovascular side effects, limiting the clinical utility of these drugs. PGE2 signals through four different receptors (EP1–EP4) and targeting individual receptor(s) may avoid these side effects, while retaining significant anticancer benefits. Here, we show that targeted inhibition of the EP1 receptor in the tumor cells and the tumor microenvironment resulted in the significant inhibition of tumor growth in vivo. Both dietary administration and direct injection of the EP1 receptor-specific antagonist, ONO-8713, effectively reduced the growth of established CT26 tumors in BALB/c mice, with suppression of the EP1 receptor in the tumor cells alone less effective in reducing tumor growth. This antitumor effect was associated with reduced Fas ligand expression and attenuated tumor-induced immune suppression. In particular, tumor infiltration by CD4+CD25+Foxp3+ regulatory T cells was decreased, whereas the cytotoxic activity of isolated splenocytes against CT26 cells was increased. F4/80+ macrophage infiltration was also decreased; however, there was no change in macrophage phenotype. These findings suggest that the EP1 receptor represents a potential target for the treatment of colon cancer.en
dc.description.sponsorshipHealth Research Board of Ireland (RP/2007/8); Irish Cancer Society (CRF12RYA)en
dc.description.statusPeer revieweden
dc.description.versionAccepted versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationO'Callaghan G, Ryan A, Neary P, O'Mahony C, Shanahan F, Houston A (2013) 'Targeting the EP1 receptor reduces fas ligand expression and increases the anti-tumor immune response in an in vivo model of colon cancer'. International Journal of Cancer, 133 (4):825-834. doi: 10.1002/ijc.28076en
dc.identifier.doi10.1002/ijc.28076
dc.identifier.endpage834en
dc.identifier.issn1097-0215
dc.identifier.issn0020-7136
dc.identifier.issued4en
dc.identifier.journaltitleInternational Journal of Canceren
dc.identifier.startpage825en
dc.identifier.urihttps://hdl.handle.net/10468/1206
dc.identifier.volume133en
dc.language.isoenen
dc.publisherWileyen
dc.rights© 2013 UICC. This is the accepted version of an article which has been published in final form in International Journal of Cancer, available at https://doi.org/10.1002/ijc.28076 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.en
dc.subjectPGE2en
dc.subjectEP1 receptoren
dc.subjectImmune suppressionen
dc.subjectCanceren
dc.titleTargeting the EP1 receptor reduces Fas ligand expression and increases the antitumor immune response in an in vivo model of colon canceren
dc.typeArticle (peer-reviewed)en
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