dc.contributor.author	Baclaocos, Janinah
dc.contributor.author	Santesmasses, Didac
dc.contributor.author	Mariotti, Marco
dc.contributor.author	Bierła, Katarzyna
dc.contributor.author	Vetick, Michael B.
dc.contributor.author	Lynch, Sharon
dc.contributor.author	McAllen, Rob
dc.contributor.author	Mackrill, John J.
dc.contributor.author	Loughran, Gary
dc.contributor.author	Guigó, Roderic
dc.contributor.author	Szpunar, Joanna
dc.contributor.author	Copeland, Paul R.
dc.contributor.author	Gladyshev, Vadim N.
dc.contributor.author	Atkins, John F.
dc.contributor.funder	Science Foundation Ireland	en
dc.contributor.funder	Irish Research Council	en
dc.contributor.funder	Spanish Ministry of Economy and Competitiveness	en
dc.contributor.funder	EMBO	en
dc.contributor.funder	National Institutes of Health	en
dc.date.accessioned	2019-10-15T05:59:08Z
dc.date.available	2019-10-15T05:59:08Z
dc.date.issued	2019-08-20
dc.description.abstract	Selenoproteins typically contain a single selenocysteine, the 21st amino acid, encoded by a context-redefined UGA. However, human selenoprotein P (SelenoP) has a redox-functioning selenocysteine in its N-terminal domain and nine selenium transporter-functioning selenocysteines in its C-terminal domain. Here we show that diverse SelenoP genes are present across metazoa with highly variable numbers of Sec-UGAs, ranging from a single UGA in certain insects, to 9 in common spider, and up to 132 in bivalve molluscs. SelenoP genes were shaped by a dynamic evolutionary process linked to selenium usage. Gene evolution featured modular expansions of an ancestral multi-Sec domain, which led to particularly Sec-rich SelenoP proteins in many aquatic organisms. We focused on molluscs, and chose Pacific oyster Magallana gigas as experimental model. We show that oyster SelenoP mRNA with 46 UGAs is translated full-length in vivo. Ribosome profiling indicates that selenocysteine specification occurs with ~5% efficiency at UGA1 and approaches 100% efficiency at distal 3′ UGAs. We report genetic elements relevant to its expression, including a leader open reading frame and an RNA structure overlapping the initiation codon that modulates ribosome progression in a selenium-dependent manner. Unlike their mammalian counterparts, the two SECIS elements in oyster SelenoP (3′UTR recoding elements) do not show functional differentiation in vitro. Oysters can increase their tissue selenium level up to 50-fold upon supplementation, which also results in extensive changes in selenoprotein expression.	en
dc.description.sponsorship	Spanish Ministry of Economy and Competitiveness ( BIO2014-57291-R); Irish Research Council (IRCLA/2019/74 n); EMBO short-term fellowship (ASTF 289-2014); National Institutes of Health ( DK117149, GM065204, AG021518, and GM077073)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Baclaocos, J., Santesmasses, D., Mariotti, M., Bierła, K., Vetick, M. B., Lynch, S., McAllen, R., Mackrill, J. J., Loughran, G., Guigó, R., Szpunar, J., Copeland, P. R., Gladyshev, V. N. and Atkins, J. F. (2019) 'Processive Recoding and Metazoan Evolution of Selenoprotein P: Up to 132 UGAs in Molluscs', Journal of Molecular Biology.,(27pp.) [In press] DOI: 10.1016/j.jmb.2019.08.007	en
dc.identifier.doi	10.1016/j.jmb.2019.08.007	en
dc.identifier.eissn	1089-8638
dc.identifier.endpage	27	en
dc.identifier.issn	0022-2836
dc.identifier.journaltitle	Journal of Molecular Biology	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/8769
dc.language.iso	en	en
dc.publisher	Academic Press	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1853/IE/Dynamic redefinition of codons: From antivirals to an essential micronutrient/	en
dc.relation.uri	https://www.sciencedirect.com/science/article/pii/S0022283619305133?via%3Dihub
dc.rights	©2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/)	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	en
dc.subject	Selenoprotein	en
dc.subject	Selenocysteine	en
dc.subject	Recoding	en
dc.subject	Dynamic redefinition	en
dc.subject	Evolution	en
dc.title	Processive recoding and metazoan evolution of selenoprotein P: Up to 132 UGAs in molluscs	en
dc.type	Article (peer-reviewed)	en

Processive recoding and metazoan evolution of selenoprotein P: Up to 132 UGAs in molluscs

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