Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration

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dc.contributor.authorBarry, Kate M.
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorKelly, Philip M.
dc.contributor.funderDepartment of Agriculture, Food and the Marineen
dc.contributor.funderTeagascen
dc.date.accessioned2017-05-15T15:44:59Z
dc.date.available2017-05-15T15:44:59Z
dc.date.issued2017-02-04
dc.date.updated2017-05-15T15:36:05Z
dc.description.abstractExtensive enzymatic hydrolysis of milk proteins in reconstituted buttermilk powder was combined with ultrafiltration to generate a phospholipid (PL) enriched fraction with maximum permeation of hydrolysed peptides. Buttermilk, naturally high in PLs, is the ideal substrate for enrichment of these bio- and techno-functionally active compounds. A 7.8 fold increase in PL was achieved in the 50 kDa retentate; 6.16 ± 0.02% total PL compared with 0.79 ± 0.01% in the starting substrate, an increase considerably greater than previously reported. Total lipid content (% dry matter) increased 6.3 fold in the retentate, 43.43 ± 0.61%, from the starting substrate, 6.84 ± 0.17%. This combined strategic approach enabled maximum enrichment of PLs with no transmission of lipid material into the permeate, 0.09 ± 0.02% total lipid, and non-detectable levels of PLs recovered in the permeate, 0.00 ± 0.01% total PL.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (FIRM), project number: 10RD/TMFRC/709); Teagasc (Teagasc PhD Walsh Fellowship)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBarry, K. M., Dinan, T. G. and Kelly, P. M. (2017) 'Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration', International Dairy Journal, 68, pp. 80-87. doi:10.1016/j.idairyj.2016.12.016en
dc.identifier.doi10.1016/j.idairyj.2016.12.016
dc.identifier.endpage87en
dc.identifier.issn0958-6946
dc.identifier.journaltitleInternational Dairy Journalen
dc.identifier.startpage80en
dc.identifier.urihttps://hdl.handle.net/10468/3970
dc.identifier.volume68en
dc.language.isoenen
dc.publisherElsevieren
dc.rights© 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectButtermilken
dc.subjectPhospholipidsen
dc.subjectEnzymatic hydrolysisen
dc.subjectPL enrichmenten
dc.titleSelective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltrationen
dc.typeArticle (peer-reviewed)en
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