Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003

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dc.contributor.author Christiaen, Steven E. A.
dc.contributor.author O'Connell Motherway, Mary
dc.contributor.author Bottacini, Francesca
dc.contributor.author Lanigan, Noreen
dc.contributor.author Casey, Pat G.
dc.contributor.author Huys, Geert
dc.contributor.author Nelis, Hans J.
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Coenye, Tom
dc.date.accessioned 2016-02-17T11:44:40Z
dc.date.available 2016-02-17T11:44:40Z
dc.date.issued 2014
dc.identifier.citation Christiaen SEA, O'Connell Motherway M, Bottacini F, Lanigan N, Casey PG, Huys G, et al. (2014) Autoinducer-2 Plays a Crucial Role in Gut Colonization and Probiotic Functionality of Bifidobacterium breve UCC2003. PLoS ONE 9(5): e98111. doi:10.1371/journal.pone.0098111
dc.identifier.volume 9 en
dc.identifier.issued 5 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/2338
dc.identifier.doi 10.1371/journal.pone.0098111
dc.description.abstract In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition. en
dc.description.sponsorship Belgian Science Policy Office (Interuniversity Attraction Poles Programme); Health Research Board (Postdoctoral Fellowship PDTM/20011/9); Irish Research Council for Science Engineering and Technology (Postgraduate Fellowship); Science Foundation Ireland (SFI Grant No. 07/CE/B1368; SFI/12/RC/2273) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2015 Christiaen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Inflammatory bowel disease en
dc.subject Lactobacillus rhamnosus GG en
dc.subject Dna-microarray data en
dc.subject Quorum sensing role en
dc.subject Caenorhabditis elegans en
dc.subject Necrotizing enterocolitis en
dc.subject Gene expression en
dc.subject Pseudomonas aeruginosa en
dc.subject Salmonella typhimurium en
dc.subject Escherichia coli en
dc.title Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 258245552
dc.internal.wokid WOS:000336858300025
dc.contributor.funder Belgian Science Policy Office
dc.contributor.funder Agentschap voor Innovatie door Wetenschap en Technologie, Belgium
dc.contributor.funder Health Research Board
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Irish Research Council for Science Engineering and Technology
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLOS ONE en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid e98111


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© 2015 Christiaen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2015 Christiaen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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