Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration

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dc.contributor.author Barry, Kate M.
dc.contributor.author Dinan, Timothy G.
dc.contributor.author Kelly, Philip M.
dc.date.accessioned 2017-05-15T15:44:59Z
dc.date.available 2017-05-15T15:44:59Z
dc.date.issued 2017-02-04
dc.identifier.citation Barry, K. M., Dinan, T. G. and Kelly, P. M. (2017) 'Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration', International Dairy Journal, 68, pp. 80-87. doi:10.1016/j.idairyj.2016.12.016 en
dc.identifier.volume 68 en
dc.identifier.startpage 80 en
dc.identifier.endpage 87 en
dc.identifier.issn 0958-6946
dc.identifier.uri http://hdl.handle.net/10468/3970
dc.identifier.doi 10.1016/j.idairyj.2016.12.016
dc.description.abstract Extensive enzymatic hydrolysis of milk proteins in reconstituted buttermilk powder was combined with ultrafiltration to generate a phospholipid (PL) enriched fraction with maximum permeation of hydrolysed peptides. Buttermilk, naturally high in PLs, is the ideal substrate for enrichment of these bio- and techno-functionally active compounds. A 7.8 fold increase in PL was achieved in the 50 kDa retentate; 6.16 ± 0.02% total PL compared with 0.79 ± 0.01% in the starting substrate, an increase considerably greater than previously reported. Total lipid content (% dry matter) increased 6.3 fold in the retentate, 43.43 ± 0.61%, from the starting substrate, 6.84 ± 0.17%. This combined strategic approach enabled maximum enrichment of PLs with no transmission of lipid material into the permeate, 0.09 ± 0.02% total lipid, and non-detectable levels of PLs recovered in the permeate, 0.00 ± 0.01% total PL. en
dc.description.sponsorship Department of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (FIRM), project number: 10RD/TMFRC/709); Teagasc (Teagasc PhD Walsh Fellowship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier en
dc.rights © 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Buttermilk en
dc.subject Phospholipids en
dc.subject Enzymatic hydrolysis en
dc.subject PL enrichment en
dc.title Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Ted Dinan, Psychiatry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: t.dinan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this item is restricted until 12 months after publication at the request of the publisher. en
dc.check.date 2018-02-04
dc.date.updated 2017-05-15T15:36:05Z
dc.description.version Accepted Version en
dc.internal.rssid 395124939
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Dairy Journal en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress t.dinan@ucc.ie en


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© 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2017 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
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