Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis

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dc.contributor.author Oliveira, Joana
dc.contributor.author Mahony, Jennifer
dc.contributor.author Hanemaaijer, Laurens
dc.contributor.author Kouwen, Thijs R.
dc.contributor.author Neve, Horst
dc.contributor.author MacSharry, John
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2017-09-26T11:39:22Z
dc.date.available 2017-09-26T11:39:22Z
dc.date.issued 2017
dc.identifier.citation Oliveira, J., Mahony, J., Hanemaaijer, L., Kouwen, T. R. H. M., Neve, H., MacSharry, J. and van Sinderen, D. (2017) 'Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis', Frontiers in Microbiology, 8, 1343 (11pp). doi: 10.3389/fmicb.2017.01343 en
dc.identifier.volume 8
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/4800
dc.identifier.doi 10.3389/fmicb.2017.01343
dc.description.abstract Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media S.A. en
dc.relation.uri http://journal.frontiersin.org/article/10.3389/fmicb.2017.01343/full
dc.rights © 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Temperate phages en
dc.subject Lysogeny en
dc.subject Chemical inductions en
dc.subject Prophage en
dc.subject Flow cytometry en
dc.subject Electron microscopy en
dc.subject Marine viruses en
dc.subject Phage en
dc.subject Bacteriophage en
dc.subject Temperate en
dc.subject Identification en
dc.subject Strains en
dc.subject TP901-1 en
dc.subject PCR en
dc.subject Populations en
dc.title Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.wokid WOS:000406321000001
dc.contributor.funder Science Foundation Ireland
dc.contributor.funder Royal DSM
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid 1343
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/


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© 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as © 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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