Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis
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Kouwen, Thijs R.
van Sinderen, Douwe
Frontiers Media S.A.
Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9.
Temperate phages , Lysogeny , Chemical inductions , Prophage , Flow cytometry , Electron microscopy , Marine viruses , Phage , Bacteriophage , Temperate , Identification , Strains , TP901-1 , PCR , Populations
Oliveira, J., Mahony, J., Hanemaaijer, L., Kouwen, T. R. H. M., Neve, H., MacSharry, J. and van Sinderen, D. (2017) 'Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis', Frontiers in Microbiology, 8, 1343 (11pp). doi: 10.3389/fmicb.2017.01343
© 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.