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| dc.contributor.author | Oliveira, Joana | |
| dc.contributor.author | Mahony, Jennifer | |
| dc.contributor.author | Hanemaaijer, Laurens | |
| dc.contributor.author | Kouwen, Thijs R. | |
| dc.contributor.author | Neve, Horst | |
| dc.contributor.author | MacSharry, John | |
| dc.contributor.author | van Sinderen, Douwe | |
| dc.date.accessioned | 2017-09-26T11:39:22Z | |
| dc.date.available | 2017-09-26T11:39:22Z | |
| dc.date.issued | 2017 | |
| dc.identifier.citation | Oliveira, J., Mahony, J., Hanemaaijer, L., Kouwen, T. R. H. M., Neve, H., MacSharry, J. and van Sinderen, D. (2017) 'Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis', Frontiers in Microbiology, 8, 1343 (11pp). doi: 10.3389/fmicb.2017.01343 | en |
| dc.identifier.volume | 8 | |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10468/4800 | |
| dc.identifier.doi | 10.3389/fmicb.2017.01343 | |
| dc.description.abstract | Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9. | en |
| dc.format.mimetype | application/pdf | en |
| dc.language.iso | en | en |
| dc.publisher | Frontiers Media S.A. | en |
| dc.relation.uri | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01343/full | |
| dc.rights | © 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | en |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Temperate phages | en |
| dc.subject | Lysogeny | en |
| dc.subject | Chemical inductions | en |
| dc.subject | Prophage | en |
| dc.subject | Flow cytometry | en |
| dc.subject | Electron microscopy | en |
| dc.subject | Marine viruses | en |
| dc.subject | Phage | en |
| dc.subject | Bacteriophage | en |
| dc.subject | Temperate | en |
| dc.subject | Identification | en |
| dc.subject | Strains | en |
| dc.subject | TP901-1 | en |
| dc.subject | PCR | en |
| dc.subject | Populations | en |
| dc.title | Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis | en |
| dc.type | Article (peer-reviewed) | en |
| dc.internal.authorcontactother | Douwe Van Sinderen, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie | en |
| dc.internal.availability | Full text available | en |
| dc.description.version | Published Version | en |
| dc.internal.wokid | WOS:000406321000001 | |
| dc.contributor.funder | Science Foundation Ireland
|
|
| dc.contributor.funder | Royal DSM
|
|
| dc.description.status | Peer reviewed | en |
| dc.identifier.journaltitle | Frontiers in Microbiology | en |
| dc.internal.IRISemailaddress | d.vansinderen@ucc.ie | en |
| dc.identifier.articleid | 1343 | |
| dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
|
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| dc.relation.project | info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/
|
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Except where otherwise noted, this item's license is described as © 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.