dc.contributor.author	Oliveira, Joana
dc.contributor.author	Mahony, Jennifer
dc.contributor.author	Hanemaaijer, Laurens
dc.contributor.author	Kouwen, Thijs R.
dc.contributor.author	Neve, Horst
dc.contributor.author	MacSharry, John
dc.contributor.author	van Sinderen, Douwe
dc.contributor.funder	Science Foundation Ireland
dc.contributor.funder	Royal DSM
dc.date.accessioned	2017-09-26T11:39:22Z
dc.date.available	2017-09-26T11:39:22Z
dc.date.issued	2017
dc.description.abstract	Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9.	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	1343
dc.identifier.citation	Oliveira, J., Mahony, J., Hanemaaijer, L., Kouwen, T. R. H. M., Neve, H., MacSharry, J. and van Sinderen, D. (2017) 'Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis', Frontiers in Microbiology, 8, 1343 (11pp). doi: 10.3389/fmicb.2017.01343	en
dc.identifier.doi	10.3389/fmicb.2017.01343
dc.identifier.issn	1664-302X
dc.identifier.journaltitle	Frontiers in Microbiology	en
dc.identifier.uri	https://hdl.handle.net/10468/4800
dc.identifier.volume	8
dc.language.iso	en	en
dc.publisher	Frontiers Media S.A.	en
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
dc.relation.project	info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/
dc.relation.uri	http://journal.frontiersin.org/article/10.3389/fmicb.2017.01343/full
dc.rights	© 2017, Oliveira, Mahony, Hanemaaijer, Kouwen, Neve, MacSharry and van Sinderen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Temperate phages	en
dc.subject	Lysogeny	en
dc.subject	Chemical inductions	en
dc.subject	Prophage	en
dc.subject	Flow cytometry	en
dc.subject	Electron microscopy	en
dc.subject	Marine viruses	en
dc.subject	Phage	en
dc.subject	Bacteriophage	en
dc.subject	Temperate	en
dc.subject	Identification	en
dc.subject	Strains	en
dc.subject	TP901-1	en
dc.subject	PCR	en
dc.subject	Populations	en
dc.title	Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis	en
dc.type	Article (peer-reviewed)	en

Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis

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