Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis
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Date
2017
Authors
Oliveira, Joana
Mahony, Jennifer
Hanemaaijer, Laurens
Kouwen, Thijs R.
Neve, Horst
MacSharry, John
van Sinderen, Douwe
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media S.A.
Published Version
Abstract
Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9.
Description
Keywords
Temperate phages , Lysogeny , Chemical inductions , Prophage , Flow cytometry , Electron microscopy , Marine viruses , Phage , Bacteriophage , Temperate , Identification , Strains , TP901-1 , PCR , Populations
Citation
Oliveira, J., Mahony, J., Hanemaaijer, L., Kouwen, T. R. H. M., Neve, H., MacSharry, J. and van Sinderen, D. (2017) 'Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis', Frontiers in Microbiology, 8, 1343 (11pp). doi: 10.3389/fmicb.2017.01343