Access to this article is restricted until 24 months after publication at the request of the publisher. Restriction lift date: 2021-01-04
Enhanced dark hydrogen fermentation of Enterobacter aerogenes/HoxEFUYH with carbon cloth
Loading...
Files
Accepted version
Date
2019-01-04
Authors
Cheng, Jun
Li, Hui
Zhang, Jiabei
Ding, Lingkan
Ye, Qing
Lin, Richen
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Published Version
Abstract
Long-range extracellular electron transfer through microbial nanowires is critical for efficient bacterial behaviors. The application of carbon cloth on the dark hydrogen fermentation using transgenic Enterobacter aerogenes (E. aerogenes/HoxEFUYH) was first proposed to enhance hydrogen production from glucose. Scanning electron microscopy images showed that the microbial nanowires between E. aerogenes/HoxEFUYH cells almost vanished due to the presence of carbon cloth. Approximately 59.1% of microorganisms concentrated in biofilms on the surface of carbon cloth, which probably promoted the intercellular electron transfer. The results from Fourier transform infrared spectra and Excitation Emission Matrix spectra indicated that carbon cloth biofilms primarily included polysaccharide and protein. Moreover, the fluorophore of biofilms (88.1%) was much higher than that of supernatant (11.9%). The analysis of soluble metabolic degradation byproducts revealed that carbon cloth selectively enhanced the acetate pathway (C6H12O6+2H2O→2CH3COOH+2CO2+4H2), but weakened the ethanol pathway (C6H12O6→2C2H5OH+2CO2). With 1.0 g/L carbon cloth, the hydrogen yield increased by 26.6% to 242 mL/g, and the corresponding peak hydrogen production rate increased by 60.3%.
Description
Keywords
Electro-conductive carbon cloth , Hydrogen fermentation , Transgenic Enterobacter aerogenes
Citation
Cheng, J., Li, H., Zhang, J., Ding, L., Ye, Q. and Lin, R. (2019) 'Enhanced dark hydrogen fermentation of Enterobacter aerogenes/HoxEFUYH with carbon cloth', International Journal of Hydrogen Energy, In Press, doi: 10.1016/j.ijhydene.2018.12.080