Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates

Nogacka, Alicja; Salazar, Nuria; Suarez, Marta; Milani, Christian; Arboleya, Silvia; Solis, Gonzalo; Fernandez, Nuria; Alaez, Lidia; Hernandez-Barranco, Ana M.; de los Reyes-Gavilan, Clara G.; Ventura, Marco; Gueimonde, Miguel

Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates

dc.contributor.author	Nogacka, Alicja
dc.contributor.author	Salazar, Nuria
dc.contributor.author	Suarez, Marta
dc.contributor.author	Milani, Christian
dc.contributor.author	Arboleya, Silvia
dc.contributor.author	Solis, Gonzalo
dc.contributor.author	Fernandez, Nuria
dc.contributor.author	Alaez, Lidia
dc.contributor.author	Hernandez-Barranco, Ana M.
dc.contributor.author	de los Reyes-Gavilan, Clara G.
dc.contributor.author	Ventura, Marco
dc.contributor.author	Gueimonde, Miguel
dc.contributor.funder	Gobierno del Principado de Asturias
dc.contributor.funder	Consejo Superior de Investigaciones Científicas
dc.contributor.funder	Ministero dell’Istruzione, dell’Università e della Ricerca
dc.contributor.funder	Ministerio de Economía y Competitividad
dc.date.accessioned	2017-09-26T11:39:22Z
dc.date.available	2017-09-26T11:39:22Z
dc.date.issued	2017
dc.description.abstract	Background: Disturbances in the early establishment of the intestinal microbiota may produce important implications for the infant's health and for the risk of disease later on. Different perinatal conditions may be affecting the development of the gut microbiota. Some of them, such as delivery mode or feeding habits, have been extensively assessed whereas others remain to be studied, being critical to identify their impact on the microbiota and, if any, to minimize it. Antibiotics are among the drugs most frequently used in early life, the use of intrapartum antimicrobial prophylaxis (IAP), present in over 30% of deliveries, being the most frequent source of exposure. However, our knowledge on the effects of IAP on the microbiota establishment is still limited. The aim of the present work was to evaluate the impact of IAP investigating a cohort of 40 full-term vaginally delivered infants born after an uncomplicated pregnancy, 18 of which were born from mothers receiving IAP. Results: Fecal samples were collected at 2, 10, 30, and 90 days of age. We analyzed the composition of the fecal microbiota during the first 3 months of life by 16S rRNA gene sequencing and quantified fecal short chain fatty acids by gas chromatography. The presence of genes for resistance to antibiotics was determined by PCR in the samples from 1-month-old infants. Our results showed an altered pattern of intestinal microbiota establishment in IAP infants during the first weeks of life, with lower relative proportions of Actinobacteria and Bacteroidetes and increased of Preoteobacteria and Firmicutes. A delay in the increase on the levels of acetate was observed in IAP infants. The analyses of specific antibiotic resistance genes showed a higher occurrence of some beta-lactamase coding genes in infants whose mothers received IAP. Conclusions: Our results indicate an effect of IAP on the establishing early microbiota during the first months of life, which represent a key moment for the development of the microbiota-induced host homeostasis. Understanding the impact of IAP in the gut microbiota development is essential for developing treatments to minimize it, favoring a proper gut microbiota development in IAP-exposed neonates.	en
dc.description.sponsorship	Ministerio de Economía y Competitividad (PCIN-2015-233; AGL2013-43770R); Consejo Superior de Investigaciones Científicas (Unit of Information Resources for Research); Plan Regional de Investigación del Principado de Asturias (GRUPIN14-043)	en
dc.description.status	Peer reviewed	en
dc.description.version	Published Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	93
dc.identifier.citation	Nogacka, A., Salazar, N., Suárez, M., Milani, C., Arboleya, S., Solís, G., Fernández, N., Alaez, L., Hernández-Barranco, A. M., de los Reyes-Gavilán, C. G., Ventura, M. and Gueimonde, M. (2017) 'Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates', Microbiome, 5(1), 93 (10pp). doi: 10.1186/s40168-017-0313-3	en
dc.identifier.doi	10.1186/s40168-017-0313-3
dc.identifier.issn	2049-2618
dc.identifier.journaltitle	Microbiome	en
dc.identifier.uri	https://hdl.handle.net/10468/4802
dc.identifier.volume	5
dc.language.iso	en	en
dc.publisher	Biomed Central Ltd	en
dc.relation.uri	https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0313-3
dc.rights	© 2017, the Authors. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.	en
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Intestinal microbiota	en
dc.subject	Microbiome	en
dc.subject	Antibiotics	en
dc.subject	Neonate	en
dc.subject	Intrapartum antimicrobial prophylaxis	en
dc.subject	Antibiotics	en
dc.subject	Infant gut microbiota	en
dc.subject	Group B streptococcus	en
dc.subject	Early life	en
dc.subject	Fecal microbiota	en
dc.subject	Children	en
dc.subject	Health	en
dc.subject	Establishment	en
dc.subject	Consequences	en
dc.subject	Colonization	en
dc.subject	Physiology	en
dc.title	Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates	en
dc.type	Article (peer-reviewed)	en