dc.contributor.author	Kuniyoshi, Taís Mayumi
dc.contributor.author	Mendonça, Carlos Miguel Nóbrega
dc.contributor.author	Borges Vieira, Viviane
dc.contributor.author	Robl, Diogo
dc.contributor.author	Gombossy de Melo Franco, Bernadette Dora
dc.contributor.author	Todorov, Svetoslav Dimitrov
dc.contributor.author	Tomé, Elisabetta
dc.contributor.author	O'Connor, Paula Mary
dc.contributor.author	Converti, Attilio
dc.contributor.author	Araújo, Welington Luiz
dc.contributor.author	Palmeiras Vasconcellos, Lívia Paula Silva
dc.contributor.author	de Mello Varani, Alessandro
dc.contributor.author	Cotter, Paul David
dc.contributor.author	Rabelo, Sarita Cândid
dc.contributor.author	de Souza Oliveira, Ricardo Pinheiro
dc.contributor.funder	Fundação de Amparo à Pesquisa do Estado de São Paulo	en
dc.contributor.funder	Conselho Nacional de Desenvolvimento Científico e Tecnológico	en
dc.contributor.funder	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	en
dc.date.accessioned	2021-08-18T09:05:37Z
dc.date.available	2021-08-18T09:05:37Z
dc.date.issued	2021-07-14
dc.date.updated	2021-08-18T08:45:43Z
dc.description.abstract	Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.	en
dc.description.sponsorship	Fundação de Amparo à Pesquisa do Estado de São Paulo (Grants 2013/07914-8, 2015/ 24777-0, 2018/04385-8 and 2018/25511-1) Conselho Nacional de Desenvolvimento Científico e Tecnológico and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance Code 001)	en
dc.description.status	Peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Kuniyoshi, T. M., Mendonça, C. M. N., Borges Vieira, V., Robl, D., Gombossy de Melo Franco, B. D., Todorov, S. D., Tomé, E., O'Connor, P. M., Converti, A., Araújo, W. L., Palmeiras Vasconcellos, L. P. S., de Mello Varani, A., Cotter, P. D., Rabelo, S. C. and de Souza Oliveira, R. P. (2021) 'Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse', Bioresource Technology, 338, 125565 (12pp). doi: 10.1016/j.biortech.2021.125565	en
dc.identifier.doi	10.1016/j.biortech.2021.125565	en
dc.identifier.endpage	12	en
dc.identifier.issn	1873-2976
dc.identifier.issn	0960-8524
dc.identifier.journaltitle	Bioresource Technology	en
dc.identifier.startpage	1	en
dc.identifier.uri	https://hdl.handle.net/10468/11752
dc.identifier.volume	338	en
dc.language.iso	en	en
dc.publisher	Elsevier Ltd.	en
dc.rights	© 2021, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.	en
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/	en
dc.subject	Sugarcane bagasse	en
dc.subject	Pediococcus pentosaceus	en
dc.subject	Bacteriocin	en
dc.subject	Hemicellulose hydrolysat	en
dc.title	Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse	en
dc.type	Article (peer-reviewed)	en

Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

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