Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

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Date
2021-07-14
Authors
Kuniyoshi, Taí­s Mayumi
Mendonça, Carlos Miguel Nóbrega
Borges Vieira, Viviane
Robl, Diogo
Gombossy de Melo Franco, Bernadette Dora
Todorov, Svetoslav Dimitrov
Tomé, Elisabetta
O'Connor, Paula Mary
Converti, Attilio
Araújo, Welington Luiz
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Elsevier Ltd.
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Abstract
Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.
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Keywords
Sugarcane bagasse , Pediococcus pentosaceus , Bacteriocin , Hemicellulose hydrolysat
Citation
Kuniyoshi, T. M., Mendonça, C. M. N., Borges Vieira, V., Robl, D., Gombossy de Melo Franco, B. D., Todorov, S. D., Tomé, E., O'Connor, P. M., Converti, A., Araújo, W. L., Palmeiras Vasconcellos, L. P. S., de Mello Varani, A., Cotter, P. D., Rabelo, S. C. and de Souza Oliveira, R. P. (2021) 'Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse', Bioresource Technology, 338, 125565 (12pp). doi: 10.1016/j.biortech.2021.125565