Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains

Varela, Javier A.; Montini, Noemi; Scully, Damhan; Van der Ploeg, Ralph; Oreb, Mislav; Boles, Eckhard; Hirota, Junya; Akada, Rinji; Hoshida, Hisashi; Morrissey, John P.

Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains

dc.contributor.author	Varela, Javier A.
dc.contributor.author	Montini, Noemi
dc.contributor.author	Scully, Damhan
dc.contributor.author	Van der Ploeg, Ralph
dc.contributor.author	Oreb, Mislav
dc.contributor.author	Boles, Eckhard
dc.contributor.author	Hirota, Junya
dc.contributor.author	Akada, Rinji
dc.contributor.author	Hoshida, Hisashi
dc.contributor.author	Morrissey, John P.
dc.contributor.funder	FP7 People: Marie-Curie Actions	en
dc.contributor.funder	Japan Science and Technology Agency	en
dc.date.accessioned	2018-06-11T12:19:54Z
dc.date.available	2018-06-11T12:19:54Z
dc.date.issued	2017-04-18
dc.date.updated	2018-06-11T08:52:22Z
dc.description.abstract	Kluyveromyces marxianus is a safe yeast used in the food and biotechnology sectors. One of the important traits that sets it apart from the familiar yeasts, Saccharomyces cerevisiae, is its capacity to grow using lactose as a carbon source. Like in its close relative, Kluyveromyces lactis, this requires lactose transport via a permease and intracellular hydrolysis of the disaccharide. Given the importance of the trait, it was intriguing that most, but not all, strains of K. marxianus are reported to consume lactose efficiently. In this study, primarily through heterologous expression in S. cerevisiae and K. marxianus, it was established that a single gene, LAC12, is responsible for lactose uptake in K. marxianus. Strains that failed to transport lactose showed variation in 13 amino acids in the Lac12p protein, rendering the protein non-functional for lactose transport. Genome analysis showed that the LAC12 gene is present in four copies in the subtelomeric regions of three different chromosomes but only the ancestral LAC12 gene encodes a functional lactose transporter. Other copies of LAC12 may be non-functional or have alternative substrates. The analysis raises some interesting questions regarding the evolution of sugar transporters in K. marxianus.	en
dc.description.sponsorship	Japan Science and Technology Agency (Advanced Low Carbon Technology R&D Program)	en
dc.description.status	Peer reviewed	en
dc.description.version	Accepted Version	en
dc.format.mimetype	application/pdf	en
dc.identifier.articleid	fox021
dc.identifier.citation	Varela, J. A., Montini, N., Scully, D., Van der Ploeg, R., Oreb, M., Boles, E., Hirota, J., Akada, R., Hoshida, H. and Morrissey, J. P. (2017) 'Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains', FEMS Yeast Research, 17(3), fox021 (13pp). doi:10.1093/femsyr/fox021	en
dc.identifier.doi	10.1093/femsyr/fox021
dc.identifier.issn	1567-1356
dc.identifier.issn	1567-1364
dc.identifier.issued	3	en
dc.identifier.journaltitle	FEMS Yeast Research	en
dc.identifier.uri	https://hdl.handle.net/10468/6273
dc.identifier.volume	17	en
dc.language.iso	en	en
dc.publisher	Oxford University Press on behalf of Federation of European Microbiological Societies	en
dc.relation.project	info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/606795/EU/Yeast Cell Factories: Training Researchers to Apply Modern Post-Genomic Methods In Yeast Biotechnology/YEASTCELL	en
dc.rights	© 2017, FEMS. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS Yeast Research following peer review. The version of record Varela, J. A., Montini, N., Scully, D., Van der Ploeg, R., Oreb, M., Boles, E., Hirota, J., Akada, R., Hoshida, H. and Morrissey, J. P. (2017) 'Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains', FEMS Yeast Research, 17(3), fox021 (13pp). doi:10.1093/femsyr/fox021] is available online at: https://doi.org/10.1093/femsyr/fox021	en
dc.subject	Lactose utilisation	en
dc.subject	LAC12	en
dc.subject	Kluyveromyces marxianus	en
dc.title	Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains	en
dc.type	Article (peer-reviewed)	en