Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies.

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dc.contributor.authorHouston, Aileen M.
dc.contributor.authorWilliams, John M.
dc.contributor.authorRovis, Tihana L.
dc.contributor.authorShanley, Daniel K.
dc.contributor.authorO'Riordan, Ronan T.
dc.contributor.authorKiely, Patrick A.
dc.contributor.authorBall, Melanie
dc.contributor.authorBarry, Orla P.
dc.contributor.authorKelly, Jacquie
dc.contributor.authorFanning, Aine
dc.contributor.authorMacSharry, John
dc.contributor.authorMandelboim, Ofer
dc.contributor.authorSinger, Bernhard B.
dc.contributor.authorJonjic, Stipan
dc.contributor.authorMoore, Thomas F.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-09-04T15:19:52Z
dc.date.available2019-09-04T15:19:52Z
dc.date.issued2016-03-18
dc.date.updated2019-09-02T06:13:54Z
dc.description.abstractPregnancy-specific glycoproteins (PSGs) are immunoglobulin superfamily members related to the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family and are encoded by 10 genes in the human. They are secreted at high levels by placental syncytiotrophoblast into maternal blood during pregnancy, and are implicated in immunoregulation, thromboregulation, and angiogenesis. To determine whether PSGs are expressed in tumors, we characterized 16 novel monoclonal antibodies to human PSG1 and used 2 that do not cross-react with CEACAMs to study PSG expression in tumors and in the gastrointestinal (GI) tract using tissue arrays and immunohistochemistry. Staining was frequently observed in primary squamous cell carcinomas and colonic adenocarcinomas and was correlated with the degree of tumor differentiation, being largely absent from metastatic samples. Staining was also observed in normal oesophageal and colonic epithelium. PSG expression in the human and mouse GI tract was confirmed using quantitative RT-PCR. However, mRNA expression was several orders of magnitude lower in the GI tract compared to placenta. Our results identify a non-placental site of PSG expression in the gut and associated tumors, with implications for determining whether PSGs have a role in tumor progression, and utility as tumor biomarkers.en
dc.description.sponsorshipScience Foundation Ireland (Principal Investigator award)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationHouston, A., Williams, J. M., Rovis, T. L., Shanley, D. K., O'Riordan, R. T., Kiely, P. A., Ball, M., Barry, O. P., Kelly, J., Fanning, A., MacSharry, J., Mandelboim, O., Singer, B. B., Jonjic, S. and Moore, T. (2016) 'Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies', mAbs, 8(3), pp. 491-500. doi: 10.1080/19420862.2015.1134410en
dc.identifier.doi10.1080/19420862.2015.1134410en
dc.identifier.endpage500en
dc.identifier.issn1942-0870
dc.identifier.issued3en
dc.identifier.journaltitlemAbsen
dc.identifier.startpage491en
dc.identifier.urihttps://hdl.handle.net/10468/8453
dc.identifier.volume8en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.relation.urihttps://www.tandfonline.com/doi/full/10.1080/19420862.2015.1134410
dc.rights© 2016 Taylor & Francis Group, LLC. This is an Accepted Manuscript of an article published by Taylor & Francis in mAbs on 18 March 2016, available online: http://www.tandfonline.com/10.1080/19420862.2015.1134410en
dc.subjectCarcinoembryonic antigenen
dc.subjectCEACAMen
dc.subjectColonen
dc.subjectColonic adenocarcinomaen
dc.subjectMonoclonal antibodyen
dc.subjectPlacentaen
dc.subjectPregnancy-specific glycoproteinen
dc.subjectPSG1en
dc.subjectOesphagus Squamous epitheliumen
dc.subjectSquamous cell carcinomaen
dc.subjectTrophoblasten
dc.titlePregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies.en
dc.typeArticle (peer-reviewed)en
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