Metabolome and microbiome profiling of a stress-sensitive rat model of gut-brain axis dysfunction

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dc.contributor.authorBassett, Shalome A.
dc.contributor.authorYoung, Wayne
dc.contributor.authorFraser, Karl
dc.contributor.authorDalziel, Julie E.
dc.contributor.authorWebster, Jim
dc.contributor.authorRyan, Leigh
dc.contributor.authorFitzgerald, Patrick
dc.contributor.authorStanton, Catherine
dc.contributor.authorDinan, Timothy G.
dc.contributor.authorCryan, John F.
dc.contributor.authorClarke, Gerard
dc.contributor.authorHyland, Niall P.
dc.contributor.authorRoy, Nicole C.
dc.contributor.funderMinistry for Business Innovation and Employmenten
dc.date.accessioned2019-10-23T10:04:16Z
dc.date.available2019-10-23T10:04:16Z
dc.date.issued2019-10-01
dc.description.abstractStress negatively impacts gut and brain health. Individual differences in response to stress have been linked to genetic and environmental factors and more recently, a role for the gut microbiota in the regulation of stress-related changes has been demonstrated. However, the mechanisms by which these factors influence each other are poorly understood, and there are currently no established robust biomarkers of stress susceptibility. To determine the metabolic and microbial signatures underpinning physiological stress responses, we compared stress-sensitive Wistar Kyoto (WKY) rats to the normo-anxious Sprague Dawley (SD) strain. Here we report that acute stress-induced strain-specific changes in brain lipid metabolites were a prominent feature in WKY rats. The relative abundance of Lactococcus correlated with the relative proportions of many brain lipids. In contrast, plasma lipids were significantly elevated in response to stress in SD rats, but not in WKY rats. Supporting these findings, we found that the greatest difference between the SD and WKY microbiomes were the predicted relative abundance of microbial genes involved in lipid and energy metabolism. Our results provide potential insights for developing novel biomarkers of stress vulnerability, some of which appear genotype specific.en
dc.description.sponsorshipMinistry for Business Innovation and Employment, New Zealand (AgResearch Strategic Science Investment Fund Contract #A21246)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid14026en
dc.identifier.citationBassett, S. A., Young, W., Fraser, K., Dalziel, J. E., Webster, J., Ryan, L., Fitzgerald, P., Stanton, C., Dinan, T. G., Cryan, J. F., Clarke, G., Hyland, N. and Roy, N. C. (2019) 'Metabolome and microbiome profiling of a stress-sensitive rat model of gut-brain axis dysfunction', Scientific Reports, 9(1), 14026. (13pp.) DOI: 10.1038/s41598-019-50593-3en
dc.identifier.doi10.1038/s41598-019-50593-3en
dc.identifier.eissn2045-2322
dc.identifier.endpage13en
dc.identifier.issued1en
dc.identifier.journaltitleScientific reportsen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/8843
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urihttps://www.nature.com/articles/s41598-019-50593-3
dc.rights© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectFat metabolismen
dc.subjectMicrobiomeen
dc.subjectStress and resilienceen
dc.titleMetabolome and microbiome profiling of a stress-sensitive rat model of gut-brain axis dysfunctionen
dc.typeArticle (peer-reviewed)en
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