Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse
Kuniyoshi, Taís Mayumi; Mendonça, Carlos Miguel Nóbrega; Borges Vieira, Viviane; Robl, Diogo; Gombossy de Melo Franco, Bernadette Dora; Todorov, Svetoslav Dimitrov; Tomé, Elisabetta; O'Connor, Paula Mary; Converti, Attilio; Araújo, Welington Luiz; Palmeiras Vasconcellos, Lívia Paula Silva; de Mello Varani, Alessandro; Cotter, Paul David; Rabelo, Sarita Cândid; de Souza Oliveira, Ricardo Pinheiro
Date:
2021-07-14
Copyright:
© 2021, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.
Full text restriction information:
Access to this article is restricted until 24 months after publication by request of the publisher.
Restriction lift date:
2023-07-14
Citation:
Kuniyoshi, T. M., Mendonça, C. M. N., Borges Vieira, V., Robl, D., Gombossy de Melo Franco, B. D., Todorov, S. D., Tomé, E., O'Connor, P. M., Converti, A., Araújo, W. L., Palmeiras Vasconcellos, L. P. S., de Mello Varani, A., Cotter, P. D., Rabelo, S. C. and de Souza Oliveira, R. P. (2021) 'Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse', Bioresource Technology, 338, 125565 (12pp). doi: 10.1016/j.biortech.2021.125565
Abstract:
Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.
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Except where otherwise noted, this item's license is described as © 2021, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.